Colony-forming unit enumeration by a plate-MPN [most probable number] method [Microbiological examination of food]

Concepts of the standard surface plate method and the most probable number method (MPN) were combined to provide a new enumeration technique (plate-MPN). Three discrete 0.01-ml samples of an appropriate decimal dilution were inoculated onto each quadrant of a pre-dried petri plate. The discrete spot...

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Veröffentlicht in:Journal of food protection 1983-10, Vol.46 (10), p.836-841
Hauptverfasser: Tan, S.T, Maxcy, R.B, Stroup, W.W
Format: Artikel
Sprache:eng
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Zusammenfassung:Concepts of the standard surface plate method and the most probable number method (MPN) were combined to provide a new enumeration technique (plate-MPN). Three discrete 0.01-ml samples of an appropriate decimal dilution were inoculated onto each quadrant of a pre-dried petri plate. The discrete spots from the inoculum were then observed for growth after incubation. Results were interpreted analogous to a 3-tube MPN test using presently available tables. Application of the test to pure cultures and mixed flora provided no evidence to indicate the plate-MPN technique to be any less accurate than the standard technique for microbial counts. The plate-MPN technique was less precise than the standard technique. However, the plate-MPN technique has many advantages over traditional methods.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028x-46.10.836