Norbornene Probes for the Detection of Cysteine Sulfenic Acid in Cells

Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superio...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:ACS chemical biology 2019-04, Vol.14 (4), p.594-598
Hauptverfasser: Alcock, Lisa J, Oliveira, Bruno L, Deery, Michael J, Pukala, Tara L, Perkins, Michael V, Bernardes, Gonçalo J. L, Chalker, Justin M
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Norbornene derivatives were validated as probes for cysteine sulfenic acid on proteins and in live cells. Trapping sulfenic acids with norbornene probes is highly selective and revealed a different reactivity profile than the traditional dimedone reagent. The norbornene probe also revealed a superior chemoselectivity when compared to a commonly used dimedone probe. Together, these results advance the study of cysteine oxidation in biological systems.
ISSN:1554-8929
1554-8937
DOI:10.1021/acschembio.8b01104