The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture
Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tigh...
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| Veröffentlicht in: | Cell biology international 2019-05, Vol.43 (5), p.456-465 |
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| creator | Buhl, Monika Kloskowski, Tomasz Jundzill, Arkadiusz Gagat, Maciej Balcerczyk, Daria Adamowicz, Jan Grzanka, Alina Nowacki, Maciej Drewa, Gerard Olszewska‐Słonina, Dorota Drewa, Tomasz Pokrywczynska, Marta |
| description | Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells (P |
| doi_str_mv | 10.1002/cbin.11109 |
| format | Article |
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The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells (P < 0.05). Meroclone‐like cells expressed higher levels of p63 compared to paraclone‐ like cells (P < 0.05). The level of Ki‐67 and PanCK for meroclone‐ and paraclone‐ like cells was comparable (P > 0.05). β1 and β4 integrins were not expressed. Expression of zonula occludens‐1 (ZO‐1) in cell‐cell junctions for paraclone‐, meroclone‐and holoclone‐like cells was 17.6 ± 0.6, 14.7 ± 0.5, and 16.1 ± 0.4, respectively. The results of actin filaments (F‐actin) expression were 253,634 ± 6,920 for meroclone‐like cells, 198,512 ± 7,977 for paraclone‐like cells and 133,544 ± 3,169 for holoclone‐like cells. Three urothelial cell types with differing features can be isolated from the bladder. Holoclone‐like cells are the richest in stem cells and should be used in further studies for construction of neo‐bladder or neo‐conduit using tissue engineering methods.</description><identifier>ISSN: 1065-6995</identifier><identifier>EISSN: 1095-8355</identifier><identifier>DOI: 10.1002/cbin.11109</identifier><identifier>PMID: 30729622</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Actin ; Bladder ; Cell culture ; Cell junctions ; Cloning ; Cytology ; Filaments ; holoclones ; Integrins ; meroclones ; Morphology ; paraclones ; regenerative medicine ; Stem cell transplantation ; Stem cells ; Tissue engineering ; Urinary bladder ; urothelial cells</subject><ispartof>Cell biology international, 2019-05, Vol.43 (5), p.456-465</ispartof><rights>2019 International Federation for Cell Biology</rights><rights>2019 International Federation for Cell Biology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3579-60adeb705bdee9405b25f92077124090722bd895e7614ba6674a55054a84d38e3</citedby><cites>FETCH-LOGICAL-c3579-60adeb705bdee9405b25f92077124090722bd895e7614ba6674a55054a84d38e3</cites><orcidid>0000-0003-3010-2561</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbin.11109$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbin.11109$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30729622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buhl, Monika</creatorcontrib><creatorcontrib>Kloskowski, Tomasz</creatorcontrib><creatorcontrib>Jundzill, Arkadiusz</creatorcontrib><creatorcontrib>Gagat, Maciej</creatorcontrib><creatorcontrib>Balcerczyk, Daria</creatorcontrib><creatorcontrib>Adamowicz, Jan</creatorcontrib><creatorcontrib>Grzanka, Alina</creatorcontrib><creatorcontrib>Nowacki, Maciej</creatorcontrib><creatorcontrib>Drewa, Gerard</creatorcontrib><creatorcontrib>Olszewska‐Słonina, Dorota</creatorcontrib><creatorcontrib>Drewa, Tomasz</creatorcontrib><creatorcontrib>Pokrywczynska, Marta</creatorcontrib><title>The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture</title><title>Cell biology international</title><addtitle>Cell Biol Int</addtitle><description>Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells (P < 0.05). Meroclone‐like cells expressed higher levels of p63 compared to paraclone‐ like cells (P < 0.05). The level of Ki‐67 and PanCK for meroclone‐ and paraclone‐ like cells was comparable (P > 0.05). β1 and β4 integrins were not expressed. Expression of zonula occludens‐1 (ZO‐1) in cell‐cell junctions for paraclone‐, meroclone‐and holoclone‐like cells was 17.6 ± 0.6, 14.7 ± 0.5, and 16.1 ± 0.4, respectively. The results of actin filaments (F‐actin) expression were 253,634 ± 6,920 for meroclone‐like cells, 198,512 ± 7,977 for paraclone‐like cells and 133,544 ± 3,169 for holoclone‐like cells. Three urothelial cell types with differing features can be isolated from the bladder. Holoclone‐like cells are the richest in stem cells and should be used in further studies for construction of neo‐bladder or neo‐conduit using tissue engineering methods.</description><subject>Actin</subject><subject>Bladder</subject><subject>Cell culture</subject><subject>Cell junctions</subject><subject>Cloning</subject><subject>Cytology</subject><subject>Filaments</subject><subject>holoclones</subject><subject>Integrins</subject><subject>meroclones</subject><subject>Morphology</subject><subject>paraclones</subject><subject>regenerative medicine</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Urinary bladder</subject><subject>urothelial cells</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kU1LxDAQhoMofl_8ARLwImLXJE3S5qiLXyB6Wc8lbadsNNusSbu6_97UqgcPwpCZDA8vM_MidETJhBLCLqrStBNKKVEbaDe-IslTITaHWopEKiV20F4IL4RQynO5jXZSkjElGdtFq9kccG2aBjy0HYaPpYcQjGuxa_ArrPFC-1fwAcdO7103B2u0xXNnXWVdq-05XoD_rXVb46X2evzjCqwN2LRDrEznHa562_UeDtBWo22Aw--8j55vrmfTu-Th6fZ-evmQVKnIVCKJrqHMiChrAMVjZqJRjGQZZZyouAUr61wJyCTlpZYy41oIIrjOeZ3mkO6j01F36d1bD6ErFiYMU-kWXB8KRhWnIuc0jejJH_TF9T5uESnGeJpKRkikzkaq8i4ED02x9CbeaF1QUgxuFIMbxZcbET7-luzLBdS_6M_5I0BH4N1YWP8jVUyv7h9H0U9AJpRI</recordid><startdate>201905</startdate><enddate>201905</enddate><creator>Buhl, Monika</creator><creator>Kloskowski, Tomasz</creator><creator>Jundzill, Arkadiusz</creator><creator>Gagat, Maciej</creator><creator>Balcerczyk, Daria</creator><creator>Adamowicz, Jan</creator><creator>Grzanka, Alina</creator><creator>Nowacki, Maciej</creator><creator>Drewa, Gerard</creator><creator>Olszewska‐Słonina, Dorota</creator><creator>Drewa, Tomasz</creator><creator>Pokrywczynska, Marta</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3010-2561</orcidid></search><sort><creationdate>201905</creationdate><title>The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture</title><author>Buhl, Monika ; Kloskowski, Tomasz ; Jundzill, Arkadiusz ; Gagat, Maciej ; Balcerczyk, Daria ; Adamowicz, Jan ; Grzanka, Alina ; Nowacki, Maciej ; Drewa, Gerard ; Olszewska‐Słonina, Dorota ; Drewa, Tomasz ; Pokrywczynska, Marta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3579-60adeb705bdee9405b25f92077124090722bd895e7614ba6674a55054a84d38e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Bladder</topic><topic>Cell culture</topic><topic>Cell junctions</topic><topic>Cloning</topic><topic>Cytology</topic><topic>Filaments</topic><topic>holoclones</topic><topic>Integrins</topic><topic>meroclones</topic><topic>Morphology</topic><topic>paraclones</topic><topic>regenerative medicine</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Tissue engineering</topic><topic>Urinary bladder</topic><topic>urothelial cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buhl, Monika</creatorcontrib><creatorcontrib>Kloskowski, Tomasz</creatorcontrib><creatorcontrib>Jundzill, Arkadiusz</creatorcontrib><creatorcontrib>Gagat, Maciej</creatorcontrib><creatorcontrib>Balcerczyk, Daria</creatorcontrib><creatorcontrib>Adamowicz, Jan</creatorcontrib><creatorcontrib>Grzanka, Alina</creatorcontrib><creatorcontrib>Nowacki, Maciej</creatorcontrib><creatorcontrib>Drewa, Gerard</creatorcontrib><creatorcontrib>Olszewska‐Słonina, Dorota</creatorcontrib><creatorcontrib>Drewa, Tomasz</creatorcontrib><creatorcontrib>Pokrywczynska, Marta</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buhl, Monika</au><au>Kloskowski, Tomasz</au><au>Jundzill, Arkadiusz</au><au>Gagat, Maciej</au><au>Balcerczyk, Daria</au><au>Adamowicz, Jan</au><au>Grzanka, Alina</au><au>Nowacki, Maciej</au><au>Drewa, Gerard</au><au>Olszewska‐Słonina, Dorota</au><au>Drewa, Tomasz</au><au>Pokrywczynska, Marta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2019-05</date><risdate>2019</risdate><volume>43</volume><issue>5</issue><spage>456</spage><epage>465</epage><pages>456-465</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells (P < 0.05). Meroclone‐like cells expressed higher levels of p63 compared to paraclone‐ like cells (P < 0.05). The level of Ki‐67 and PanCK for meroclone‐ and paraclone‐ like cells was comparable (P > 0.05). β1 and β4 integrins were not expressed. Expression of zonula occludens‐1 (ZO‐1) in cell‐cell junctions for paraclone‐, meroclone‐and holoclone‐like cells was 17.6 ± 0.6, 14.7 ± 0.5, and 16.1 ± 0.4, respectively. The results of actin filaments (F‐actin) expression were 253,634 ± 6,920 for meroclone‐like cells, 198,512 ± 7,977 for paraclone‐like cells and 133,544 ± 3,169 for holoclone‐like cells. Three urothelial cell types with differing features can be isolated from the bladder. Holoclone‐like cells are the richest in stem cells and should be used in further studies for construction of neo‐bladder or neo‐conduit using tissue engineering methods.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30729622</pmid><doi>10.1002/cbin.11109</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-3010-2561</orcidid></addata></record> |
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| subjects | Actin Bladder Cell culture Cell junctions Cloning Cytology Filaments holoclones Integrins meroclones Morphology paraclones regenerative medicine Stem cell transplantation Stem cells Tissue engineering Urinary bladder urothelial cells |
| title | The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture |
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