The different expression of key markers on urothelial holoclonal, meroclonal, and paraclonal cells in in vitro culture

Urothelial cell populations which differ in morphology and proliferation capacities can be isolated from the urinary bladder. The goal of this study was to analyze a clonal, proliferative, and self‐renewing potential of porcine urothelial cells and to compare expression of selected adhesion and tight junction molecules, urothelial and stem cell markers for the urothelial clone types. Urothelial cells were isolated from 10 porcine urinary bladders. Three different clone types: holoclone‐, meroclone‐and paraclone‐like colonies were identified based on their morphology. To characterize and compare the urothelial clones the immunofluorescent stains were performed. Expression of pancytokeratin (PanCK), Ki‐67 and p63 was higher for holoclone‐ like cells compared to meroclone‐and paraclone‐like cells (P