Human Endothelial Colony Forming Cells Express Intracellular CD133 that Modulates their Vasculogenic Properties

Stem cells at the origin of endothelial progenitor cells and in particular endothelial colony forming cells (ECFCs) subtype have been largely supposed to be positive for the CD133 antigen, even though no clear correlation has been established between its expression and function in ECFCs. We postulated that CD133 in ECFCs might be expressed intracellularly, and could participate to vasculogenic properties. ECFCs extracted from cord blood were used either fresh ( n = 4) or frozen (n = 4), at culture days