Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux
Aims The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species. Methods and Results Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junctio...
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| Veröffentlicht in: | Journal of applied microbiology 2019-06, Vol.126 (6), p.1891-1898 |
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| container_issue | 6 |
| container_start_page | 1891 |
| container_title | Journal of applied microbiology |
| container_volume | 126 |
| creator | Kayar Dogan, E. Abaci Gunyar, O. Topal, F. Alper, E. Ekinci, N. |
| description | Aims
The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species.
Methods and Results
Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real‐time PCR‐based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P |
| doi_str_mv | 10.1111/jam.14250 |
| format | Article |
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The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species.
Methods and Results
Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real‐time PCR‐based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P < 0·005).
Conclusion
While there was no difference between the bacterial load in the two groups, variation was observed in bacterial species. Most of the bacteria identified in distal oesophagus of the patients with gastroesophageal reflux were Gram negative.
Significance and Impact of the Study
The human oesophagus was considered sterile until quite recently. Molecular techniques displayed the presence of a diverse bacterial species in the oesophagus. Although it is known that dysbiosis in the oesophagus causes GERD, and that Barrett's oesophagus can trigger the development of oesophageal adenocarcinoma, its etiopathogenesis is not clear. A limited number of published studies support the importance of the present study.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14250</identifier><identifier>PMID: 30873693</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenocarcinoma ; Bacteria ; bacterial load ; bacterial species ; Barrett's esophagus ; Biopsy ; distal oesophagus ; Dysbacteriosis ; Esophageal cancer ; Esophagitis ; Gastric cancer ; gastric reflux ; Gastroesophageal reflux ; real‐time PCR ; Species</subject><ispartof>Journal of applied microbiology, 2019-06, Vol.126 (6), p.1891-1898</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3130-a51b15f85ee4a967af54fa6c09a425d778664f56477e457ea1d6540b6ef1d8443</cites><orcidid>0000-0002-4033-1862</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14250$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14250$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30873693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kayar Dogan, E.</creatorcontrib><creatorcontrib>Abaci Gunyar, O.</creatorcontrib><creatorcontrib>Topal, F.</creatorcontrib><creatorcontrib>Alper, E.</creatorcontrib><creatorcontrib>Ekinci, N.</creatorcontrib><title>Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species.
Methods and Results
Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real‐time PCR‐based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P < 0·005).
Conclusion
While there was no difference between the bacterial load in the two groups, variation was observed in bacterial species. Most of the bacteria identified in distal oesophagus of the patients with gastroesophageal reflux were Gram negative.
Significance and Impact of the Study
The human oesophagus was considered sterile until quite recently. Molecular techniques displayed the presence of a diverse bacterial species in the oesophagus. Although it is known that dysbiosis in the oesophagus causes GERD, and that Barrett's oesophagus can trigger the development of oesophageal adenocarcinoma, its etiopathogenesis is not clear. A limited number of published studies support the importance of the present study.</description><subject>Adenocarcinoma</subject><subject>Bacteria</subject><subject>bacterial load</subject><subject>bacterial species</subject><subject>Barrett's esophagus</subject><subject>Biopsy</subject><subject>distal oesophagus</subject><subject>Dysbacteriosis</subject><subject>Esophageal cancer</subject><subject>Esophagitis</subject><subject>Gastric cancer</subject><subject>gastric reflux</subject><subject>Gastroesophageal reflux</subject><subject>real‐time PCR</subject><subject>Species</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kU1PGzEQhq0KVCBw6B-oLHEphw32-is50qiFIhAXOFsT7yxxtFkva69C_j1OAhyQOhePZh6_mpmXkB-cjXmOyyWsxlyWin0jx1xoVZTalAe7XBaKmfKInMS4ZIwLpvR3ciTYxAg9Fcfk9Te4hL2HhsYOncdIoa1oCilX5p-9JkBFfUvTAmnl47YZMIZuAc9D3DY6SB7bFOnap8VOYpuEIVHX-Na7_OEZYuq9oz3WzfB6Sg5raCKevb8j8vT3z-Psprh7uP43u7ornMjTFqD4nKt6ohAlTLWBWskatGNTyAtXxky0lrXS0hiUyiDwSivJ5hprXk2kFCPya6_b9eFlwJjsykeHTQMthiHakk8F14rna4zI-Rd0GYa-zdPZMocQhjOVqYs95foQY17Gdr1fQb-xnNmtHTbbYXd2ZPbnu-IwX2H1SX7cPwOXe2DtG9z8X8neXt3vJd8AdDSUOA</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Kayar Dogan, E.</creator><creator>Abaci Gunyar, O.</creator><creator>Topal, F.</creator><creator>Alper, E.</creator><creator>Ekinci, N.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4033-1862</orcidid></search><sort><creationdate>201906</creationdate><title>Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux</title><author>Kayar Dogan, E. ; Abaci Gunyar, O. ; Topal, F. ; Alper, E. ; Ekinci, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3130-a51b15f85ee4a967af54fa6c09a425d778664f56477e457ea1d6540b6ef1d8443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenocarcinoma</topic><topic>Bacteria</topic><topic>bacterial load</topic><topic>bacterial species</topic><topic>Barrett's esophagus</topic><topic>Biopsy</topic><topic>distal oesophagus</topic><topic>Dysbacteriosis</topic><topic>Esophageal cancer</topic><topic>Esophagitis</topic><topic>Gastric cancer</topic><topic>gastric reflux</topic><topic>Gastroesophageal reflux</topic><topic>real‐time PCR</topic><topic>Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kayar Dogan, E.</creatorcontrib><creatorcontrib>Abaci Gunyar, O.</creatorcontrib><creatorcontrib>Topal, F.</creatorcontrib><creatorcontrib>Alper, E.</creatorcontrib><creatorcontrib>Ekinci, N.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kayar Dogan, E.</au><au>Abaci Gunyar, O.</au><au>Topal, F.</au><au>Alper, E.</au><au>Ekinci, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-06</date><risdate>2019</risdate><volume>126</volume><issue>6</issue><spage>1891</spage><epage>1898</epage><pages>1891-1898</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species.
Methods and Results
Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real‐time PCR‐based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P < 0·005).
Conclusion
While there was no difference between the bacterial load in the two groups, variation was observed in bacterial species. Most of the bacteria identified in distal oesophagus of the patients with gastroesophageal reflux were Gram negative.
Significance and Impact of the Study
The human oesophagus was considered sterile until quite recently. Molecular techniques displayed the presence of a diverse bacterial species in the oesophagus. Although it is known that dysbiosis in the oesophagus causes GERD, and that Barrett's oesophagus can trigger the development of oesophageal adenocarcinoma, its etiopathogenesis is not clear. A limited number of published studies support the importance of the present study.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30873693</pmid><doi>10.1111/jam.14250</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-4033-1862</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-06, Vol.126 (6), p.1891-1898 |
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| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete |
| subjects | Adenocarcinoma Bacteria bacterial load bacterial species Barrett's esophagus Biopsy distal oesophagus Dysbacteriosis Esophageal cancer Esophagitis Gastric cancer gastric reflux Gastroesophageal reflux real‐time PCR Species |
| title | Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux |
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