Bacterial species and total bacterial load in the distal oesophagus in patients with and without clinical gastric reflux
Aims The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species. Methods and Results Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junctio...
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Veröffentlicht in: | Journal of applied microbiology 2019-06, Vol.126 (6), p.1891-1898 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Aims
The purpose of this study is to compare distal oesophagus of persons with and without gastric reflux in terms of bacterial load and presence of certain bacterial species.
Methods and Results
Two biopsy specimens were obtained from the distal oesophagus at 5 cm above the gastroesophageal junction of each of the 50 patients (20 with normal oesophagus and 30 with reflux oesophagitis) under endoscopic examination and used for histological examination and DNA isolation. We used a real‐time PCR‐based assay to quantify the bacterial load and the presence of certain bacterial species from one of the biopsy samples. The biopsy specimens taken from the patients with reflux oesophagitis were consistent with gastroesophageal reflux disease (GERD). The bacterial load did not significantly differ between the groups (P |
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ISSN: | 1364-5072 1365-2672 |
DOI: | 10.1111/jam.14250 |