Hairpin probes based click polymerization for label-free electrochemical detection of human T-lymphotropic virus types II

A novel, simple, and label-free amplification electrochemical impedance method for quantitative detection of Human T-lymphotropic virus types II(HTLV-II) via click chemistry-mediated of hairpin DNA probes (hairpins) with polymers was developed. The hairpins were firstly attached to the gold electrod...

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Veröffentlicht in:Analytica chimica acta 2019-06, Vol.1059, p.86-93
Hauptverfasser: Cheng, Di, Zhang, Yaping, Wen, Dongxiao, Guo, Zhuangzhuang, Yang, Huaixia, Liu, Yanju, Kong, Jinming
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Sprache:eng
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Zusammenfassung:A novel, simple, and label-free amplification electrochemical impedance method for quantitative detection of Human T-lymphotropic virus types II(HTLV-II) via click chemistry-mediated of hairpin DNA probes (hairpins) with polymers was developed. The hairpins were firstly attached to the gold electrode surface by an S-Au bond, the azido terminals of hairpins were close to the electrode surface, which make it difficult to be approached. After hybridizing with HTLV-II, the hairpins were unfolded and experienced a big configuration change, which made the azido terminals of the hairpins available to conjugate with alkynyl-containing polymer, called P(DEB-DSDA), formed by 1,4-diacetylenebenzene (DEB) and 4,4′-Diazido-2,2′-stilbenedisulfonic acid disodium salt tetrahydrate (DSDA) via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). With amount of P(DEB-DSDA) conjugated with the hairpin probes via click polymerization, its electrochemical signal can have a great amplification. Under optimized experimental conditions, this new probe showed a low detection limit of 0.171 pM with a good liner in the range of 1 pM-1 nM. Meanwhile, the biosensor also exhibited good selectivity and reliability in detection of real serum samples, indicating that it has great application potential in clinical DNA diagnosis and detection. A novel, simple, and label-free amplification electrochemical impedance method for quantitative detection of Human T-lymphotropic virus types II via click chemistry-mediated of hairpin DNA probes (hairpins) with polymers was developed. [Display omitted] •In absence of enzymes and nanomaterials, a novel and label-free amplification electrochemical impedance technique for the detection of HTLV-II was developed.•The DNA detection based on click-chemistry EIS amplification has considerable simplicity, label-free, cheap “green” process, highly selective and flexibility with respect to reporter design.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2019.01.027