Biomonitoring of bisphenols A, F, S in human milk and probabilistic risk assessment for breastfed infants

The present study addresses the presence of bisphenols A (BPA) and its analogs bisphenol F (BPF) and S (BPS) in milk of 120 mothers living in Valencia (Spain) and participating in the BETTERMILK project (year 2015). We also studied the factors that could influence the BPA levels and estimated the exposure and the risk for breast fed infants. The frequency of detection of total (conjugated + unconjugated) and unconjugated-BPA were 83% and 77%, with a geometric mean of 0.29 ng/mL and 0.15 ng/mL, respectively. The frequency of detection was much lower for total-BPF (22%) and total-BPS (1.1%). The place of residence of the mother and the use of personal care products showed significant association with BPA concentrations. The estimated daily intake of total-BPA for breastfed infants amounted to a geometric mean of 0.04 μg/kg bw and a 95th percentile of 1.0 μg/kg bw, below the tolerable daily intake of 4 μg/kg bw-day established by EFSA. To our knowledge, this is the largest biomonitoring study of bisphenols in human milk in Europe. [Display omitted] •BPA, BPS and BPF levels were determined in human milk samples from Spanish mothers.•DF of total and u-BPA were 83 and 77%, and a GM of 0.29 and 0.15 ng/mL, respectively.•BPF and BPS present low frequencies of detection on the studied population.•Levels of u-BPA do not change during lactation.•Studied infants BPA estimated daily intake was below EFSA t-TDI of 4 μg/kg bw.