Assessment of endocrine disruptor effects of levonorgestrel and its photoproducts: Environmental implications of released fractions after their photocatalytic removal

•The paper coupled photodegradation and endocrine disruption assay in environment risk assessment.•UV-A applied in photo degradation of steroidal hormones is not enough to reduce it from wastewater.•The photocatalytic process applying UV-C wavelength reduce levonorgestrel (LNG) from water but unknown photoproducts may arise.•Degraded fraction of LNG discharged to water affluent may produce more complex and diverse effects.•β-hCG hormone may be used in reproductive endocrine disruptor assessment for waterborne substances. The presence of levonorgestrel (LNG) in water bodies via direct discharge and human excretion has been reported worldwide, but its effects on the reproduction of aquatic species and humans are still unknown. Owing to its recalcitrant properties, LNG is not completely removed during wastewater treatment plants, and many species may be exposed to low traces of this compound from discharged effluents. Thus, in this study, a photocatalytic process for removing LNG along with screening of endocrine disruptor effects for risk assessment was applied. Although the removal rate of LNG by ultraviolet C (UV-C) radiation was >90%, reproductive toxicity testing using the BeWo cell line exposed to LNG and its degraded fraction showed the reduced production of basal human chorionic gonadotropin hormone (β-hCG) by more than 73%, from 8.90 mIU mL−1 to