Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers
Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screen...
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| Veröffentlicht in: | Plant disease 1998-12, Vol.82 (12), p.1319-1322 |
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| creator | Yi, H.Y Rufty, R.C Wernsman, E.A |
| description | Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screened with 1,500 random decamers. A low rate of genetic polymorphism ( |
| doi_str_mv | 10.1094/PDIS.1998.82.12.1319 |
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Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screened with 1,500 random decamers. A low rate of genetic polymorphism (<10%) was detected among these lines. Two populations (F1 and F3) of maternally derived doubled haploid (MDH) lines of burley tobacco, developed from the cross NC 528 x Ky 14, were used to map the Rk gene. NC 528, Ky 14, three Rk-resistant (Rk-R) DNA bulks, and three Rk-susceptible (Rk-S) bulks generated from F1-derived MDH individuals were screened with the primers that amplified bands polymorphic between Rk-R and Rk-S lines. A total of 67 F1MDH lines and 59 F3MDH lines were screened with the primers that amplified bands polymorphic between Rk-R bulks and Rk-S bulks to confirm linkage between candidate markers and the Rk gene. Sixteen RAPD markers were positioned at six loci in a map 24.1 centimorgans long. Six RAPD markers, including one identified in the F3MDH population, were mapped at the Rk locus.</description><identifier>ISSN: 0191-2917</identifier><identifier>EISSN: 1943-7692</identifier><identifier>DOI: 10.1094/PDIS.1998.82.12.1319</identifier><identifier>PMID: 30845463</identifier><identifier>CODEN: PLDIDE</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; bulked segregant analysis ; chromosome mapping ; Fundamental and applied biological sciences. Psychology ; genetic resistance ; genetic techniques and protocols ; Genetics and breeding of economic plants ; linkage (genetics) ; Meloidogyne incognita ; Nicotiana tabacum ; Pest animals ; Pest resistance ; plant parasitic nematodes ; random amplified polymorphic DNA technique ; segregation ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Plant disease, 1998-12, Vol.82 (12), p.1319-1322</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-5b8c530ff03f64d7f502df53d2ea7359e88c13e2a60e035910626581281287783</citedby><cites>FETCH-LOGICAL-c404t-5b8c530ff03f64d7f502df53d2ea7359e88c13e2a60e035910626581281287783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3711,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1670524$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30845463$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yi, H.Y</creatorcontrib><creatorcontrib>Rufty, R.C</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><title>Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers</title><title>Plant disease</title><addtitle>Plant Dis</addtitle><description>Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screened with 1,500 random decamers. A low rate of genetic polymorphism (<10%) was detected among these lines. Two populations (F1 and F3) of maternally derived doubled haploid (MDH) lines of burley tobacco, developed from the cross NC 528 x Ky 14, were used to map the Rk gene. NC 528, Ky 14, three Rk-resistant (Rk-R) DNA bulks, and three Rk-susceptible (Rk-S) bulks generated from F1-derived MDH individuals were screened with the primers that amplified bands polymorphic between Rk-R and Rk-S lines. A total of 67 F1MDH lines and 59 F3MDH lines were screened with the primers that amplified bands polymorphic between Rk-R bulks and Rk-S bulks to confirm linkage between candidate markers and the Rk gene. Sixteen RAPD markers were positioned at six loci in a map 24.1 centimorgans long. Six RAPD markers, including one identified in the F3MDH population, were mapped at the Rk locus.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>bulked segregant analysis</subject><subject>chromosome mapping</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic resistance</subject><subject>genetic techniques and protocols</subject><subject>Genetics and breeding of economic plants</subject><subject>linkage (genetics)</subject><subject>Meloidogyne incognita</subject><subject>Nicotiana tabacum</subject><subject>Pest animals</subject><subject>Pest resistance</subject><subject>plant parasitic nematodes</subject><subject>random amplified polymorphic DNA technique</subject><subject>segregation</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><issn>0191-2917</issn><issn>1943-7692</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNpFkE1vEzEQhi0EoqHwDxD4wKEcNow_1z5WLR-ViohaerYc7zhdkqyD7Qjx73GUANIrWR49M_Y8hLxmMGdg5YfF9c39nFlr5obPWYtg9gmZMStF12vLn5IZMMs6bll_Rl6U8gMApNTmOTkTYKSSWszI4qvf7cZpResj0pxS7dZTqnTCra9paCUsY6l-CkhXOCG9uFu_p-NEa1r6EBL9NdZHene5uKZbn9eYy0vyLPpNwVen85w8fPr4_epLd_vt883V5W0XJMjaqaUJSkCMIKKWQx8V8CEqMXD0vVAWjQlMIPcaENqdgeZaGcYP6XsjzsnFce4up597LNVtxxJws_ETpn1xnBmrNOOcN1Qe0ZBTKRmj2-Wxffe3Y-AOLt3BpTu4dIY71tJctrY3pxf2yy0O_5r-ymvAuxPgS_CbmJumsfwfrntQXDbs7RGLPjm_yg15uOfABHBjTVtN_AFx4oMm</recordid><startdate>19981201</startdate><enddate>19981201</enddate><creator>Yi, H.Y</creator><creator>Rufty, R.C</creator><creator>Wernsman, E.A</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19981201</creationdate><title>Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers</title><author>Yi, H.Y ; Rufty, R.C ; Wernsman, E.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-5b8c530ff03f64d7f502df53d2ea7359e88c13e2a60e035910626581281287783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>bulked segregant analysis</topic><topic>chromosome mapping</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic resistance</topic><topic>genetic techniques and protocols</topic><topic>Genetics and breeding of economic plants</topic><topic>linkage (genetics)</topic><topic>Meloidogyne incognita</topic><topic>Nicotiana tabacum</topic><topic>Pest animals</topic><topic>Pest resistance</topic><topic>plant parasitic nematodes</topic><topic>random amplified polymorphic DNA technique</topic><topic>segregation</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yi, H.Y</creatorcontrib><creatorcontrib>Rufty, R.C</creatorcontrib><creatorcontrib>Wernsman, E.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yi, H.Y</au><au>Rufty, R.C</au><au>Wernsman, E.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers</atitle><jtitle>Plant disease</jtitle><addtitle>Plant Dis</addtitle><date>1998-12-01</date><risdate>1998</risdate><volume>82</volume><issue>12</issue><spage>1319</spage><epage>1322</epage><pages>1319-1322</pages><issn>0191-2917</issn><eissn>1943-7692</eissn><coden>PLDIDE</coden><abstract>Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screened with 1,500 random decamers. A low rate of genetic polymorphism (<10%) was detected among these lines. Two populations (F1 and F3) of maternally derived doubled haploid (MDH) lines of burley tobacco, developed from the cross NC 528 x Ky 14, were used to map the Rk gene. NC 528, Ky 14, three Rk-resistant (Rk-R) DNA bulks, and three Rk-susceptible (Rk-S) bulks generated from F1-derived MDH individuals were screened with the primers that amplified bands polymorphic between Rk-R and Rk-S lines. A total of 67 F1MDH lines and 59 F3MDH lines were screened with the primers that amplified bands polymorphic between Rk-R bulks and Rk-S bulks to confirm linkage between candidate markers and the Rk gene. Sixteen RAPD markers were positioned at six loci in a map 24.1 centimorgans long. Six RAPD markers, including one identified in the F3MDH population, were mapped at the Rk locus.</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>30845463</pmid><doi>10.1094/PDIS.1998.82.12.1319</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant disease, 1998-12, Vol.82 (12), p.1319-1322 |
| issn | 0191-2917 1943-7692 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences bulked segregant analysis chromosome mapping Fundamental and applied biological sciences. Psychology genetic resistance genetic techniques and protocols Genetics and breeding of economic plants linkage (genetics) Meloidogyne incognita Nicotiana tabacum Pest animals Pest resistance plant parasitic nematodes random amplified polymorphic DNA technique segregation Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers |
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