Mapping the root-knot nematode resistance gene (Rk) in tobacco with RAPD markers

Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screen...

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Veröffentlicht in:	Plant disease 1998-12, Vol.82 (12), p.1319-1322
Hauptverfasser:	Yi, H.Y, Rufty, R.C, Wernsman, E.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences bulked segregant analysis chromosome mapping Fundamental and applied biological sciences. Psychology genetic resistance genetic techniques and protocols Genetics and breeding of economic plants linkage (genetics) Meloidogyne incognita Nicotiana tabacum Pest animals Pest resistance plant parasitic nematodes random amplified polymorphic DNA technique segregation Varietal selection. Specialized plant breeding, plant breeding aims
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Beschreibung
Zusammenfassung:	Random amplified polymorphic DNA (RAPD) analysis was conducted to map the Rk gene in tobacco which conditions resistance to races 1 and 3 of the root-knot nematode, Meloidogyne incognita. Resistant burley tobacco genotype NC 528, containing the Rk gene, and the susceptible cultivar Ky 14 were screened with 1,500 random decamers. A low rate of genetic polymorphism (
ISSN:	0191-2917 1943-7692
DOI:	10.1094/PDIS.1998.82.12.1319