Effects of titania nanotube surfaces on osteogenic differentiation of human adipose-derived stem cells

The surface of an implant is important for successful osseointegration and long-term stability as it can aid in cell migration and proliferation, cell differentiation and allow extracellular matrix production. Earlier studies have shown that nanostructuring the surface of titanium can enhance mesenchymal stem cell (MSC) migration, proliferation, and differentiation. Although many studies have evaluated MSC response on nanostructured surfaces, there are only a few studies that have explored the response of adipose-derived stem cells (ADSC) on titania nanotube surfaces. Because ADSC exhibit great potential in regenerative medicine and have already proven effective in developing new treatments, this study aims to further understand how ADSC interact with titania nanotube surfaces. The results of this study indicate that titania nanotube surfaces enhance ADSC proliferation and differentiation that is also dependent on the size of nanotubes. Additionally, the favorable response of ADSC on nanotube surfaces suggests a potential application in orthopedic tissue regeneration. The goal of this study was to better understand the effect of titania nanotubes (NT) on the proliferation and differentiation of human adipose-derived stem cells (ADSC). Different NT surfaces were fabricated using anodization. Adipose derived stem cells (ADSC) were cultured for these surfaces for one week to evaluate cell adhesion and proliferation. The ADSC were then differentiated for 4 weeks to evaluate differentiation markers such as alkaline phosphatase activity, calcium deposition and osteocalcin expression. [Display omitted]