Knockdown of ribosomal protein S3 causes preimplantation developmental arrest in mice

Ribosomal protein S3 (RpS3), a member of the ribosome 40S subunit, has conventional ribosomal function and additional extraribosomal functions. The aim of the present study was to analyze the expression and localization of RpS3 and its function in early embryogenesis in mice. RpS3 mRNA and protein were expressed in multiple mouse tissues. In the ovary, RpS3 protein was ubiquitously and highly expressed in oocytes and granulosa cells. After ovulation and fertilization, RpS3 mRNA and protein were detected in oocytes and preimplantation embryos. Furthermore, RpS3 protein was localized in the cytoplasm of oocytes and preimplantation embryos. Moreover, knockdown of RpS3 in zygotes led to a significantly decreased rate of blastocyst formation. These results provide the first evidence for a novel function of RpS3 in regulating early embryonic development in mice. •RpS3 protein was highly expressed in granulosa cells, oocytes and preimplantation embryos.•RpS3 protein was localized in the cytoplasm of oocytes and preimplantation embryos.•Knockdown of RpS3 in zygotes led to a significantly decreased rate of blastocyst formation. RpS3 protein has the novel function of involvement in early embryonic development in mice.