Ornamental morphology of ionic liquid functionalized ternary doped N, P, F and N, B, F-reduced graphene oxide and their prevention activities of bacterial biofilm-associated with orthopedic implantation

The multifunctional biological active material design for bone tissue engineering is essential to induce osteoblast cell proliferation and attachment. Adhesion of bacteria on biomaterials to produce biofilms can be major contributors to the pathogenesis of implant material associated infections. This research work focuses on NPF& NBF elemental doping and functionalization of reduced graphene oxide using an imidazolium-based ionic liquid such as BMIM PF6 and BMIM BF4 by hydrothermal method. The resulting tri doped reduced graphene oxide (NPF-rGO and NBF-rGO) composite was further used as a scaffold for bone tissue engineering and anti-biofilm activities. The observation of the effect of NPF-rGO and NBF-rGO on the morphology, adhesion and cell proliferation of HOS cell was investigated. Moreover, the tri doped composite tested its antibiofilm properties against B. subtilis, E. coli, K. pneumoniae, and P. aeruginosa pathogenic bacteria. In-vitro studies clearly show the effectiveness of N, P, B, and F doping promoting the rGO mineralization, biocompatibility, and destruction of bacterial biofilm formation. The result of this study suggests that NPF-rGO and NBF-rGO hybrid material will be a promising scaffold for bone reaeration and implantation with a minimal bacterial infection. [Display omitted] •Nano-sphere and cube shaped NPF & NBF tri doped rGO was prepared by the assistance of BMIM PF6 and BMIM BF4 ionic liquid.•Ionic liquid has been acted as a functionalizing as well as templating agent with hetero atoms (N, P, B. F) source.•The structural and morphological modification of rGO with respect to ILs has been systematically investigated.•The cell viability and Morphological studies designated a significant proliferation of HOS during the culture time.