Acuticoccus sediminis sp. nov., isolated from deep-sea sediment of the Indian Ocean and proposal of Acuticoccaceae fam. nov

A Gram-staining negative, aerobic, oval-shaped bacterium, designated strain PTG4-2 , was isolated from deep-sea sediment of the Indian Ocean. Growth was observed with 1-9 % (w/v) NaCl with optimal growth with 3 %, at pH 6.0-10.0 with an optimum of pH 7.0, and at 4-40 °C with an optimum of 30 °C. Positive for catalase and oxidase. The results of a 16S rRNA gene sequence comparison indicated that PTG4-2 was most closely related to Acuticoccus yangtzensis JL1095 (97.3 %), followed by Acuticoccus kandeliae J103 (96.5 %), all other species shared 5 %) were summed feature 8 [C18 : 1ω7c/ω6c (72.2 %)], C18 : 0 (8.4 %), C20 : 1ω7c (6.4 %) and C16 : 0 (6.3 %). The polar lipids consisted of phosphatidylglycerol, three unidentified phospholipids, two unidentified glycolipids, one unidentified aminolipid and one unknown lipid. The DNA G+C content of PTG4-2 is 69.2 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, PTG4-2 should be classified as representing a novel species of the genus Acuticoccus, for which the name Acuticoccus sediminis sp. nov. is proposed, with the type strain PTG4-2 (=MCCC 1A01274 =KCTC 52323 ). In addition, a novel family, Acuticoccaceae fam. nov., is proposed to accommodate the genus Acuticoccus.