Effects of s-metolachlor on early life stages of marbled crayfish

The effects of s-metolachlor chronic exposure at concentrations of 1.1 μg/L (maximal real environmental concentration in the Czech Republic), 11 μg/L (environmental relevant concentration) and 110 μg/L on early life stages of marbled crayfish (Procambarus virginalis) was evaluated under laboratory conditions. All s-metolachlor exposures resulted in higher mortality, delay ontogenetic development with accompanied slower growth and excited behaviour (increase of total distance moved and walking speed). Significantly lower superoxide dismutase, catalase, glutathione S-transferase activity and reduced glutathione level was observed at two higher tested concentrations (11 and 110 μg/L) of s-metolachlor compared with the control. S-metolachlor in concentrations 110 μg/L showed alteration of the tubular system of hepatopancreas including focal disintegration of tubular epithelium and notable reduction in epithelial cells number, especially B-cells. In conclusion, potential risk associated with using of s-metolachlor in agriculture, due to effects on non-target aquatic organisms as documented on early life stages of marbled crayfish in this study, should be taken into account. [Display omitted] •How s-metolachlor herbicide, have effects on early life stages of crayfish.•S-metolachlor caused delay in ontogenetic development and reduced growth.•Exposure of s-metolachlor caused histopathological changes in gills of crayfish.•S-metolachlor affected behaviour and antioxidant system.