Identification and functional prediction of circRNAs in Populus Euphratica Oliv. heteromorphic leaves

Populus euphratica Oliv. has typical heterophylly. Linear, lanceolate, ovate and broad-ovate leaves appeared in turn from sprouting to development, to maturity. The environmental adaptabilities of P. euphraticas with different leaves were also different. To explore the role of circRNAs on the morphogenesis of P. euphratica heteromorphic leaves (P.hl) and their stress response, the expression profile of circRNAs was analyzed by strand-specific RNA sequencing for the above four kinds of heteromorphic leaves. According to ceRNA hypothesis, 18 differentially expressed cirRNAs (DECs) could influence the expression of 84 mRNAs by antagonizing 23 miRNAs in five sample-pairs. Based on the function of 84 mRNAs, these DECs participate in development process, response to stimulus, response to hormonal et al. Therefore, these circRNAs were involved in the P.hl morphogenesis and stress response by interacting with miRNAs and mRNAs. Our study complemented the genebank of P. euphratica and provided a new strategy for studying leaf development. •The desert plant P. euphratica has typical heterophylly.•The molecular mechanism of the morphogenesis of P. euphratica heteromorphic leaves (P.hl) is still unclear.•CircRNAs were identified for the first time in P.hl.•CircRNAs of P.hl were involved in the P.hl morphogenesis and stress response by interacting with miRNAs and mRNAs.