Macroalgae to nanoparticles: Study of Ulva lactuca L. role in biosynthesis of gold and silver nanoparticles and of their cytotoxicity on colon cancer cell lines
Marine bio-resources are being widely studied as an invaluable source of compounds with therapeutic applicability. In particular, macroalgae contain an extended variety of bioactive compounds with different structures and promising biological applications. In this work, Ulva lactuca L. (hereafter UL...
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Veröffentlicht in: | Materials Science & Engineering C 2019-04, Vol.97, p.498-509 |
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Sprache: | eng |
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Zusammenfassung: | Marine bio-resources are being widely studied as an invaluable source of compounds with therapeutic applicability. In particular, macroalgae contain an extended variety of bioactive compounds with different structures and promising biological applications. In this work, Ulva lactuca L. (hereafter UL) was utilyzed for the synthesis of gold and silver nanoparticles. Full characterization by UV–Vis spectroscopy, TEM, HRTEM and STEM miscroscopies, Z Potential and FTIR spectroscopy was performed. The first time in the scientific literature, the composition of carbohydrates of UL extract and their changes observed after nanoparticles synthesis were explored in order to investigate their possible role in the biosynthetic process. The reducing power, total phenolic content and DPPH scavenging activity of UL extract, Au@UL and Ag@UL nanoparticles were determined. The effects of UL extract, Au@UL and Ag@UL were tested in vitro on the colon cancer cell lines HT-29 and Caco-2, on normal primary neonatal dermal fibroblast cell line PCS-201-010, as well as on normal colon cell line CCD-112CoN. Lastly, the apoptotic activity and cellular uptake evaluation was determined for Au@UL and Ag@UL.
•Aqueous extract of Ulva lactuca served to synthesize gold and silver nanoparticles.•The composition of the extract before and after the synthesis was determined.•This provided new information about the possible role of sugars during the synthesis.•The effects of UL extract, Au@UL and Ag@UL were tested in vitro on colon cancer cells.•Apoptotic activity and cellular uptake evaluation was determined for Au@UL and Ag@UL. |
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ISSN: | 0928-4931 1873-0191 |
DOI: | 10.1016/j.msec.2018.12.066 |