An MAP kinase interacts with LHK1 and regulates nodule organogenesis in Lotus japonicus
Symbiosis receptor-like kinase (SymRK) is a key protein mediating the legume- Rhizobium symbiosis. Our previous work has identified an MAP kinase kinase, SIP2, as a SymRK-interacting protein to positively regulate nodule organogenesis in Lotus japonicus , suggesting that an MAPK cascade might be inv...
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Veröffentlicht in: | Science China. Life sciences 2019-09, Vol.62 (9), p.1203-1217 |
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Sprache: | eng |
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Zusammenfassung: | Symbiosis receptor-like kinase (SymRK) is a key protein mediating the legume-
Rhizobium
symbiosis. Our previous work has identified an MAP kinase kinase, SIP2, as a SymRK-interacting protein to positively regulate nodule organogenesis in
Lotus japonicus
, suggesting that an MAPK cascade might be involved in
Rhizobium
-legume symbiosis. In this study, LjMPK6 was identified as a phosphorylation target of SIP2. Stable transgenic
L. japonicus
with RNAi silencing of
LjMPK6
decreased the numbers of nodule primordia (NP) and nodule, while plants overexpressing
LjMPK6
increased the numbers of nodule, infection threads (ITs), and NP, indicating that LjMPK6 plays a positive role in nodulation. LjMPK6 could interact with a cytokinin receptor, LHK1 both
in vivo
and
in vitro
. LjMPK6 was shown to compete with LHP1 to bind to the receiver domain (RD) of LHK1and to downregulate the expression of two
LjACS
(1-aminocyclopropane-1-carboxylic acid synthase) genes and ethylene levels during nodulation. This study demonstrated an important role of LjMPK6 in regulation of nodule organogenesis and ethylene production in
L. japonicus
. |
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ISSN: | 1674-7305 1869-1889 |
DOI: | 10.1007/s11427-018-9444-9 |