Integration of a field effect transistor-based aptasensor under a hydrophobic membrane for bioelectronic nose applications

A new bioelectronic nose based on a field effect transistor coupled with an aptamer as the sensing element was developed. The gas-to-liquid extraction interface required for appropriate aptamer function was integrated into standard CMOS technology. It was developed with the use of a sacrificial aluminium etching technique combined with surface modifications by silanes for wettability control. As a proof of concept, aptamer Van74 for vanillin was immobilized on the sensitive surface of the ISFET. The developed microsystem can selectively detect vanillin vapor in a concentration range from 2.7 ppt to 0.3 ppm, with a detection limit of 2.7 ppt. The sensor was able to detect vanillin in a gas sample obtained from roasted coffee beans. This outcome provides a foundation for developing a new generation of bioelectronic noses for the detection and discrimination of volatile compounds. •A novel bioelectronic nose based on the aptamer and ISFET was introduced.•Fabrication of a gas-to-liquid extraction interface was integrated in standard CMOS-technology.•The fabricated bioelectronic nose allowed selective detection of vanillin in coffee aroma.