Characterization of cysteinyl leukotriene-related receptors and their interactions in a mouse model of asthma

•The level of P2Y12R is upregulated in the lung tissues and inflammatory cells, and increased even more robustly compared to that of CysLTR1 and CysLTR2 in the asthma mouse model.•Montelukast, HAMI 3379 showed the cross-inhibitory effect on the expression levels of CysLTR1, CysLTR2, and P2Y12R. The administration of clopidogrel suppressed mostly the expression of P2Y12R.•Montelukast significantly inhibited eosinophilic inflammation compare to clopidogrel, in contrast clopidogrel inhibited the level of Th2 interleukins rather than montelukast. Identification of the characterization of cysteinyl leukotrienes receptor (CysLTRs) could facilitate our understanding of these receptors’ role in asthma. We aimed to investigate the localization and interactions of CysLTRs using a mouse model of asthma. BALB/c mice were administered ovalbumin (OVA) to induce allergic asthma. Some mice were administered the antagonists of CysLTR1, CysLTR2, and purinergic receptor P2Y12 (P2Y12R) (montelukast, HAMI 3379 and clopidogrel, respectively). The expression levels of CysLTR1, CysLTR2, and P2Y12R on lung tissues and inflammatory cells were evaluated by western blot, flow cytometry, and immunochemistry. CysLTR1 and P2Y12R were significantly up-regulated in lung tissues (P