Binding Reaction Sites to Polysiloxanes: Unique Fluorescent Probe for Reversible Detection of ClO–/GSH Pair and the in Situ Imaging in Live Cells and Zebrafish

PDMS is biocompatible, economically viable, transparent, and facile to handle and thus is suitable for fluorescent microscopy and biological research. However, there has been no report about polysiloxane-based fluorescent probes applied in bioimaging. In this report, a two-photon polysiloxane-based reversible luminescent probe (P1) was fabricated for the first time. P1 is a powerful tool for detecting the ClO–/GSH cycle in situ both in live cells and in zebrafish. This work demonstrates the potential of polysiloxane-based fluorescent probes for versatile in vivo or in vitro applications in the future.