Extraction optimisation, purification and major antioxidant component of red pigments extracted from Camellia japonica
► We got the optimised extraction conditions of red pigments (RP) from Camellia japonica. ► The resin LX-68 was appropriate for purifying the pigments from C. japonica. ► The red pigments showed high DPPH and hydroxyl radical scavenging activities. ► Following activity-oriented separation, (−)-epica...
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Veröffentlicht in: | Food chemistry 2011-11, Vol.129 (2), p.660-664 |
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Sprache: | eng |
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Zusammenfassung: | ► We got the optimised extraction conditions of red pigments (RP) from Camellia japonica. ► The resin LX-68 was appropriate for purifying the pigments from C. japonica. ► The red pigments showed high DPPH and hydroxyl radical scavenging activities. ► Following activity-oriented separation, (−)-epicatechin was isolated as an active principle.
The optimised extraction conditions of red pigments (RP) from Camellia japonica obtained with an orthogonal design L9(34) were solid/liquid ratio, temperature, pH and extraction time as 1/10, 60°C, 1.5 and 4h, respectively. The RP were then purified by the macroporous resin method, which showed the resin LX-68 was appropriate for purifying the pigments from C. japonica. The antioxidant activities of these pigments were also investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and hydroxyl radical in vitro model systems. The DPPH scavenging activity of pigment extract was comparable to that of standard butylated hydroxyanisole (BHA). The IC50 values of the RP and BHA were 4.55 and 4.17μgml−1, respectively. The pigments showed higher hydroxyl radical-scavenging activities than that of mannitol at the same concentration. Following activity-oriented separation, (−)-epicatechin was isolated as an active principle, which exhibited excellent DPPH free radical scavenging activities with IC50 5.08μgml−1. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2011.05.001 |