Coffea arabica and C. canephora discrimination in roasted and ground coffee from reference material candidates by real-time PCR

To produce specific desirable coffee blends, Coffea arabica and C. canephora are mixed each other, in some cases to suit consumer preference, but in others to reduce production costs. In this scenario, the aim of this work was to evaluate standard candidate reference materials (RMc) for analysis of different blends of roasted and ground coffee. For this purpose, we analyzed different percentages of C. arabica and C. canephora (100:0; 50:50; 25:75; and 0:100, respectively). These RMc samples were developed in a previous study with green coffee beans submitted to medium roasting. In this work, coffee species differentiation (C. arabica and C. canephora) was analyzed by real-time PCR, using specific primers previously developed, called ARA primers. The RMc material with 100% C. canephora did not present amplification, in contrast with the samples containing C. arabica, which all presented amplification. These results indicate the specificity of ARA primers for C. arabica and that the detection system assay can be used as a promising molecular tool to identify and quantify percentages of C. arabica in different coffee blends. [Display omitted] •Evaluation of a candidate reference material for determination of the composition of coffee blends by molecular techniques.•Detection of the main commercial coffee species in blends of roasted and ground coffee by real-time PCR.•Differentiation of Coffea arabica and C. canephora in roasted and ground coffee blends.•Adaptation of the DNA extraction protocol to determine the composition of roasted and ground coffee samples by real-time PCR.•Analysis of the authenticity of the composition of coffee blends.