Suppression of microRNA‐141 suppressed p53 to protect against neural apoptosis in epilepsy by SIRT1 expression

We investigated that microRNA (miRNA)‐141 protects against epilepsy‐induced apoptosis and its reaction mechanism. The serum expression of miRNA‐141 in epilepsy model mice and control volunteer was measured by quantitative reverse‐transcription polymerase chain reaction. We found that miRNA‐141 serum expression was upregulated in patients with epilepsy. Overexpression of miRNA‐141 induced nerve cell apoptosis, suppressed proliferation, promoted caspase‐3/9, Bax and p53 protein expression, and reduced silent information regulator 1 (SIRT1) protein expression in vitro model. In addition, the downexpression miRNA‐141 using si‐miRNA‐141 reduced nerve cell apoptosis and increased proliferation, suppressed caspase‐3/9, Bax and p53 protein expression, induced SIRT1 protein expression. SIRT1 inhibitor (nicotinamide) decreased SIRT1, reduced the effects of miRNA‐141 on nerve cell apoptosis in vitro model of epilepsy through SIRT1/p53. SIRT1 agonist also reduced the effects of miRNA‐141 overexpression on nerve cell apoptosis in vitro model of epilepsy through SIRT1/p53. Our preliminary findings indicate that anti‐miRNA‐141 protects against epilepsy‐induced apoptosis via SIRT1/p53 expression.