Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines
Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stabili...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2019-06, Vol.188 (2), p.491-497 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Samsonova, Jeanne V. Saushkin, Nikolay Yu Osipov, Alexander P. Kondakov, Sergey E. Fomina, Svetlana N. Mischenko, Alexey V. |
| description | Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing. |
| doi_str_mv | 10.1007/s12010-018-02938-3 |
| format | Article |
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The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-018-02938-3</identifier><identifier>PMID: 30536031</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antibodies ; Biochemistry ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Enzyme-linked immunosorbent assay ; Foot & mouth disease ; Immunoglobulins ; Pretreatment ; Serotypes ; Strip ; Vaccination ; Viruses</subject><ispartof>Applied biochemistry and biotechnology, 2019-06, Vol.188 (2), p.491-497</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, (2018). 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Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.</description><subject>Antibodies</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Foot & mouth disease</subject><subject>Immunoglobulins</subject><subject>Pretreatment</subject><subject>Serotypes</subject><subject>Strip</subject><subject>Vaccination</subject><subject>Viruses</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc1u1DAURi0EotPCC7BAltiwqME_ceIs004LlQZ1MVW3kWPfFFcTO9gO0rwEz4yHKSCxYGXZ99zPvj4IvWH0A6O0-ZgYp4wSyhShvBWKiGdoxaRsD1v2HK0obwThXLUn6DSlR0oZV7J5iU4ElaKmgq3QjzVkMNkFj8OIO5_dEKyDhLsH7XzK-DqETLS35EtY8le8dgl0Anzv4pLwFmLI-_mAn-NbXDDcJacJw8MeX21uth12Hm9zdDNZRwcWb_U07wo_xjDhe22M8zqX84vw3XlIr9CLUe8SvH5az9Dd9dXd5Weyuf10c9ltiKkYz4SJtq1sLQyooVLQKCsBLKMKqKK2HkWZnDHBrRJja5lqh8HIqmHCSFEpLc7Q-2PsHMO3BVLuJ5cM7HbaQ1hSz8svslo2dVPQd_-gj2GJvjzuQAlJG9nyQvEjZWJIKcLYz9FNOu57RvuDrP4oqy-y-l-yelGa3j5FL8ME9k_LbzsFEEcglZJ_gPj37v_E_gTv7p0w</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Samsonova, Jeanne V.</creator><creator>Saushkin, Nikolay Yu</creator><creator>Osipov, Alexander P.</creator><creator>Kondakov, Sergey E.</creator><creator>Fomina, Svetlana N.</creator><creator>Mischenko, Alexey V.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0670-0951</orcidid></search><sort><creationdate>20190601</creationdate><title>Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines</title><author>Samsonova, Jeanne V. ; 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| subjects | Antibodies Biochemistry Biotechnology Chemistry Chemistry and Materials Science Enzyme-linked immunosorbent assay Foot & mouth disease Immunoglobulins Pretreatment Serotypes Strip Vaccination Viruses |
| title | Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines |
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