Detection of Antibodies Against Foot-and-Mouth Disease Virus Serotypes A, O and Asia-1 by ELISA in Strip-Dried Samples from Vaccinated Bovines

Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stabili...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Applied biochemistry and biotechnology 2019-06, Vol.188 (2), p.491-497
Hauptverfasser: Samsonova, Jeanne V., Saushkin, Nikolay Yu, Osipov, Alexander P., Kondakov, Sergey E., Fomina, Svetlana N., Mischenko, Alexey V.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Antibodies against foot-and-mouth disease virus serotypes A, O and Asia-1 were detected by ELISA in liquid and strip-dried samples from vaccinated bovines. The results showed high concordance of the results in both types of serum samples (coefficient of correlation varied from 0.89 to 0.96). Stability studies showed that anti-foot-and-mouth disease virus antibodies can be detected in strip-dried serum samples stored at different temperatures on a level of that in native samples. Preliminary study in strip-dried whole blood samples demonstrated good potential of this sample pretreatment procedure for the following anti-foot-and-mouth virus antibodies testing.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-018-02938-3