Intestinal Microbiota Is Altered in Patients with Gastric Cancer from Shanxi Province, China
Background Many diseases have been associated with intestinal microbial dysbiosis. Host–microbial interactions regulate immune function, which influences the development of gastric cancer. Aims The aims were to investigate the characteristics of intestinal microbiota composition in gastric cancer pa...
Gespeichert in:
Veröffentlicht in: | Digestive diseases and sciences 2019-05, Vol.64 (5), p.1193-1203 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Background
Many diseases have been associated with intestinal microbial dysbiosis. Host–microbial interactions regulate immune function, which influences the development of gastric cancer.
Aims
The aims were to investigate the characteristics of intestinal microbiota composition in gastric cancer patients and correlations between the intestinal microbiota and cellular immunity.
Methods
Fecal samples were collected from 116 gastric cancer patients and 88 healthy controls from Shanxi Province, China. The intestinal microbiota was investigated by 16S rRNA gene sequencing. Peripheral blood samples were also collected from the 66 gastric cancer patients and 46 healthy controls. The populations of peripheral T lymphocyte subpopulations and NK cells were analyzed by flow cytometry.
Results
The intestinal microbiota in gastric cancer patients was characterized by increased species richness, decreased butyrate-producing bacteria, and the enrichment of other symbiotic bacteria, especially
Lactobacillus
,
Escherichia,
and
Klebsiella
.
Lactobacillus
and
Lachnospira
were key species in the network of gastric cancer-associated bacterial genera. The combination of the genera
Lachnospira
,
Lactobacillus
,
Streptococcus
,
Veillonella,
and
Tyzzerella_3
showed good performance in distinguishing gastric cancer patients from healthy controls. There was no significant difference in enterotype distribution between healthy controls and gastric cancer patients. The percentage of CD3
+
T cells was positively correlated with the abundance of
Lactobacillus
and
Streptococcus
, and CD3
+
T cells, CD4
+
T cells, and NK cells were associated with Lachnospiraceae taxa.
Conclusions
Our study revealed a dysbiotic intestinal microbiota in gastric cancer patients. The abundance of some intestinal bacterial genera was correlated with the population of peripheral immune cells. |
---|---|
ISSN: | 0163-2116 1573-2568 |
DOI: | 10.1007/s10620-018-5411-y |