Growth hormone during in vitro fertilization in older women modulates the density of receptors in granulosa cells, with improved pregnancy outcomes

To study the effect of aging and granulosa cell growth hormone receptor (GHR) expression, and the effect of growth hormone (GH) co-treatment during IVF on receptor expression. Laboratory study. University. A total of 445 follicles were collected from 62 women undergoing standard infertility treatment. Preovulatory ovarian follicle biopsies of granulosa cells and follicular fluid. Older women with a poor ovarian reserve were co-treated with GH to determine the effect of the adjuvant during IVF on the granulosal expression density of FSH receptor (FSHR), LH receptor (LHR), bone morphogenetic hormone receptor (BMPR1B), and GHR. Ovarian reserve, granulosa cell receptor density, oocyte quality, and pregnancy and live birth rates were determined. Growth hormone co-treatment increased the receptor density for granulosal FSHR, BMPR1B, LHR, and GHR compared with the non–GH-treated patients of the same age and ovarian reserve. Growth hormone co-treatment increased GHR density, which may increase GHR activity. The GH co-treatment was associated with a significant increase in pregnancy rate. Growth hormone co-treatment restored the preovulatory down-regulation of FSHR, BMPR1B, and LHR density of the largest follicles, which may improve the maturation process of luteinization in older patients with reduced ovarian reserve. The fertility of the GH-treated patients improved. La hormona de crecimiento durante la fecundación in vitro de mujeres añosasmodula la densidad de los receptores en células de la granulosa con mejores resultados de embarazo Estudiar el efecto del envejecimiento y la expresión del receptor de la hormona de crecimiento en células de la granulosa (GHR) y el efecto del co-tratamiento con hormona del crecimiento (GH) durante la FIV en la expresión del receptor. estudio de laboratorio. Universidad. Se recolectaron un total de 445 folículos de 62 mujeres que se sometieron a un tratamiento estándar para la infertilidad. Biopsias de células de la granulosa y líquido folicular de ovarios preovulatorios. mujeres añosas con una reserva ovárica deficiente fueron tratadas conjuntamente con GH para determinar el efecto adyuvante durante la FIV en la densidad de expresión granular del receptor de FSH (FSHR), receptor de LH (LHR), receptor de hormona morfogenética ósea (BMPR1B), y GHR. La reserva ovárica, la densidad de receptores de células de la granulosa, la calidad de los ovocitos y el embarazo y las tasas de nacimientos vivos fueron determinado. El co-tratamien