Application of iTRAQ proteomics in identification of the differentially expressed proteins of placenta of pregnancy with preeclampsia

Application of iTRAQ proteomics in identification of the differentially expressed proteins of placenta of pregnancy with preeclampsia

Objective To explore the differential protein profile of preeclampsia and identify its potential biomarker. Methods Around 20 pregnant women with preeclampsia (preeclampsia group) and 20 normal‐term pregnancy (normal group) were collected from 2017 to 2018 in the study. Total protein of placenta tis...

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Veröffentlicht in:Journal of cellular biochemistry 2019-04, Vol.120 (4), p.5409-5416
Hauptverfasser: Feng, Yaling, He, Yue, Wang, Jianxia, Yuan, Hua, Zou, Jinfang, Yang, Lan, Xu, Jianjuan
Format: Artikel
Sprache:eng
Schlagworte:
Biomarkers
Deoxidizing
Identification methods
iTRAQ
Placenta
Pre-eclampsia
Preeclampsia
Pregnancy
Proteins
Proteomics
Quantitation
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Zusammenfassung:Objective To explore the differential protein profile of preeclampsia and identify its potential biomarker. Methods Around 20 pregnant women with preeclampsia (preeclampsia group) and 20 normal‐term pregnancy (normal group) were collected from 2017 to 2018 in the study. Total protein of placenta tissues was extracted, denaturized, deoxidized, and enzymolyzed. The sample was labeled with isobaric tags for relative and absolute quantitation (iTRAQ) and analyzed with mass spectrum to identify differentially expressed proteins. Results There were 37 proteins, which were differentially expressed with significance (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.27819