Mechanical Properties of Membrane Surface of Cultured Astrocyte Revealed by Atomic Force Microscopy
In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, b...
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Veröffentlicht in: | JPN J APPL PHYS PART 1 REGUL PAP SHORT NOTE REV PAP 2000-06, Vol.39 (6S), p.3711-3716 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | In order to examine the mechanical properties of the membrane surface of
astrocytes, we observed living astrocytes by atomic force microscopy (AFM)
both in contact mode and force-mapping mode.
Ridge-like structures reflecting actin filaments were observed in the
topographic images in contact mode, but not in force-mapping mode, using a
zero-loading force.
When we measured the elasticity of astrocytes, we observed that the cell membrane
above the nucleus was soft and the cell membrane above the cytosol was stiff.
In particular, the parts reflecting actin filaments were very stiff.
This effect of actin filaments on the elasticity of astrocytes was confirmed
by the loss of actin filaments after application of actin-polymerization inhibitor. |
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ISSN: | 0021-4922 1347-4065 |
DOI: | 10.1143/JJAP.39.3711 |