Cross-linked glucose isomerase crystals as a liquid chromatographic separation material
Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ p) and pore size distribution of the CLGI crystals were measured with size exclusion chromato...
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| Veröffentlicht in: | Enzyme and microbial technology 2000-04, Vol.26 (7), p.550-558 |
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| container_title | Enzyme and microbial technology |
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| creator | Pastinen, Ossi Jokela, Jouni Eerikäinen, Tero Schwabe, Tatjana Leisola, Matti |
| description | Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ
p) and pore size distribution of the CLGI crystals were measured with size exclusion chromatography using D
2O and polyethylene glycols as probes. CLGI material was capable of separating |
| doi_str_mv | 10.1016/S0141-0229(00)00137-X |
| format | Article |
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p) and pore size distribution of the CLGI crystals were measured with size exclusion chromatography using D
2O and polyethylene glycols as probes. CLGI material was capable of separating <1000 g/mol polyethylene glycols. Fifty two percent (ϵ
p = 0.47) of the total crystal volume was in pores. Pore size measurement showed that CLGI crystals were microporous material, having an average apparent pore diameter of 29 ± 0.08 Å. CLGI material separated n-alcohols C
1 to C
8 based on the hydrophobic interaction between the protein material and the carbon chain of the alcohols. Height equivalent to a theoretical plate (HETP, in millimeters) ranged from 1.6 to 0.89 for the C
1 to C
7 n-alcohol series. Despite the large crystal size, CLGI as a chirally active phase effectively separated
d- and
l-arabitol (R
s = 0.58) and showed potential for chiral separation of amino acids.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/S0141-0229(00)00137-X</identifier><identifier>PMID: 10771059</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Biological and medical sciences ; Biotechnology ; Chiral chromatography ; Cross-linking ; Crosslinking ; Crystals ; Enzyme crystals ; Fundamental and applied biological sciences. Psychology ; Glucose ; Liquid chromatography ; Methods. Procedures. Technologies ; Others ; Phase separation ; Polyethylene glycols ; Pore size ; Porosity ; Size exclusion chromatography ; Various methods and equipments</subject><ispartof>Enzyme and microbial technology, 2000-04, Vol.26 (7), p.550-558</ispartof><rights>2000 Elsevier Science Inc.</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-dff4f2cce26a45f40aaf19f6d0324f7c6092145181e7e02121d5fb33b45063ea3</citedby><cites>FETCH-LOGICAL-c423t-dff4f2cce26a45f40aaf19f6d0324f7c6092145181e7e02121d5fb33b45063ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0141-0229(00)00137-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1332161$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10771059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pastinen, Ossi</creatorcontrib><creatorcontrib>Jokela, Jouni</creatorcontrib><creatorcontrib>Eerikäinen, Tero</creatorcontrib><creatorcontrib>Schwabe, Tatjana</creatorcontrib><creatorcontrib>Leisola, Matti</creatorcontrib><title>Cross-linked glucose isomerase crystals as a liquid chromatographic separation material</title><title>Enzyme and microbial technology</title><addtitle>Enzyme Microb Technol</addtitle><description>Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ
p) and pore size distribution of the CLGI crystals were measured with size exclusion chromatography using D
2O and polyethylene glycols as probes. CLGI material was capable of separating <1000 g/mol polyethylene glycols. Fifty two percent (ϵ
p = 0.47) of the total crystal volume was in pores. Pore size measurement showed that CLGI crystals were microporous material, having an average apparent pore diameter of 29 ± 0.08 Å. CLGI material separated n-alcohols C
1 to C
8 based on the hydrophobic interaction between the protein material and the carbon chain of the alcohols. Height equivalent to a theoretical plate (HETP, in millimeters) ranged from 1.6 to 0.89 for the C
1 to C
7 n-alcohol series. Despite the large crystal size, CLGI as a chirally active phase effectively separated
d- and
l-arabitol (R
s = 0.58) and showed potential for chiral separation of amino acids.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chiral chromatography</subject><subject>Cross-linking</subject><subject>Crosslinking</subject><subject>Crystals</subject><subject>Enzyme crystals</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose</subject><subject>Liquid chromatography</subject><subject>Methods. Procedures. Technologies</subject><subject>Others</subject><subject>Phase separation</subject><subject>Polyethylene glycols</subject><subject>Pore size</subject><subject>Porosity</subject><subject>Size exclusion chromatography</subject><subject>Various methods and equipments</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkE2LFDEQhoMo7rj6E5Q-iKyH1qp8dE-fFhn8ggUPKu4t1KQru9HuzmzSLey_N7MzqCeFQIXieauKR4inCK8QsHn9GVBjDVJ2ZwAvAVC19eU9scJ129XQQXdfrH4jJ-JRzt-hUFrDQ3GC0LYIpluJb5sUc66HMP3gvroaFhczVyHHkROVn0u3eaYhV1ReNYSbJfSVu05xpDleJdpdB1dl3lGiOcSpKm1OgYbH4oEvMX5yrKfi67u3XzYf6otP7z9u3lzUTks117332kvnWDakjddA5LHzTQ9Kat-6BjqJ2uAauWWQKLE3fqvUVhtoFJM6FS8Oc3cp3iycZzuG7HgYaOK4ZFvSqgENBTz7J4hr0ykJYExBzQF1ezeJvd2lMFK6tQh2L9_eybd7sxbA3sm3lyX37Lhi2Y7c_5U62C7A8yNA2dHgE00u5D-cUhIbLNj5AeMi7mfgZLMLPDnuQ2I32z6G_1zyCysaoPs</recordid><startdate>20000401</startdate><enddate>20000401</enddate><creator>Pastinen, Ossi</creator><creator>Jokela, Jouni</creator><creator>Eerikäinen, Tero</creator><creator>Schwabe, Tatjana</creator><creator>Leisola, Matti</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000401</creationdate><title>Cross-linked glucose isomerase crystals as a liquid chromatographic separation material</title><author>Pastinen, Ossi ; Jokela, Jouni ; Eerikäinen, Tero ; Schwabe, Tatjana ; Leisola, Matti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-dff4f2cce26a45f40aaf19f6d0324f7c6092145181e7e02121d5fb33b45063ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chiral chromatography</topic><topic>Cross-linking</topic><topic>Crosslinking</topic><topic>Crystals</topic><topic>Enzyme crystals</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Liquid chromatography</topic><topic>Methods. Procedures. Technologies</topic><topic>Others</topic><topic>Phase separation</topic><topic>Polyethylene glycols</topic><topic>Pore size</topic><topic>Porosity</topic><topic>Size exclusion chromatography</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pastinen, Ossi</creatorcontrib><creatorcontrib>Jokela, Jouni</creatorcontrib><creatorcontrib>Eerikäinen, Tero</creatorcontrib><creatorcontrib>Schwabe, Tatjana</creatorcontrib><creatorcontrib>Leisola, Matti</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pastinen, Ossi</au><au>Jokela, Jouni</au><au>Eerikäinen, Tero</au><au>Schwabe, Tatjana</au><au>Leisola, Matti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cross-linked glucose isomerase crystals as a liquid chromatographic separation material</atitle><jtitle>Enzyme and microbial technology</jtitle><addtitle>Enzyme Microb Technol</addtitle><date>2000-04-01</date><risdate>2000</risdate><volume>26</volume><issue>7</issue><spage>550</spage><epage>558</epage><pages>550-558</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ
p) and pore size distribution of the CLGI crystals were measured with size exclusion chromatography using D
2O and polyethylene glycols as probes. CLGI material was capable of separating <1000 g/mol polyethylene glycols. Fifty two percent (ϵ
p = 0.47) of the total crystal volume was in pores. Pore size measurement showed that CLGI crystals were microporous material, having an average apparent pore diameter of 29 ± 0.08 Å. CLGI material separated n-alcohols C
1 to C
8 based on the hydrophobic interaction between the protein material and the carbon chain of the alcohols. Height equivalent to a theoretical plate (HETP, in millimeters) ranged from 1.6 to 0.89 for the C
1 to C
7 n-alcohol series. Despite the large crystal size, CLGI as a chirally active phase effectively separated
d- and
l-arabitol (R
s = 0.58) and showed potential for chiral separation of amino acids.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>10771059</pmid><doi>10.1016/S0141-0229(00)00137-X</doi><tpages>9</tpages></addata></record> |
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| ispartof | Enzyme and microbial technology, 2000-04, Vol.26 (7), p.550-558 |
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| subjects | Biological and medical sciences Biotechnology Chiral chromatography Cross-linking Crosslinking Crystals Enzyme crystals Fundamental and applied biological sciences. Psychology Glucose Liquid chromatography Methods. Procedures. Technologies Others Phase separation Polyethylene glycols Pore size Porosity Size exclusion chromatography Various methods and equipments |
| title | Cross-linked glucose isomerase crystals as a liquid chromatographic separation material |
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