Cross-linked glucose isomerase crystals as a liquid chromatographic separation material

Cross-linked glucose isomerase crystals as a liquid chromatographic separation material

Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ p) and pore size distribution of the CLGI crystals were measured with size exclusion chromato...

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Veröffentlicht in:Enzyme and microbial technology 2000-04, Vol.26 (7), p.550-558
Hauptverfasser: Pastinen, Ossi, Jokela, Jouni, Eerikäinen, Tero, Schwabe, Tatjana, Leisola, Matti
Format: Artikel
Sprache:eng
Schlagworte:
Biological and medical sciences
Biotechnology
Chiral chromatography
Cross-linking
Crosslinking
Crystals
Enzyme crystals
Fundamental and applied biological sciences. Psychology
Glucose
Liquid chromatography
Methods. Procedures. Technologies
Others
Phase separation
Polyethylene glycols
Pore size
Porosity
Size exclusion chromatography
Various methods and equipments
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Zusammenfassung:Cross-linked crystals of glucose isomerase (CLGI) were characterized as a liquid chromatographic separation material. The experiments were done with crystals having an average diameter of 83 μm. Porosity (ϵ p) and pore size distribution of the CLGI crystals were measured with size exclusion chromatography using D 2O and polyethylene glycols as probes. CLGI material was capable of separating
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(00)00137-X