Two-photon micro-spectroscopy of chloroplasts from Arabidopsis thaliana

Two-photon micro-spectroscopy of chloroplasts from Arabidopsis thaliana

In this study, two-photon excitation (TPE) under a microscope was employed to obtain the spectrum of chloroplast. It was found that the protoplasts from Arabidopsis thaliana may exhibit different fluorescence spectra depending on whether chloroplast exist in the cells. Further, it was observed that...

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Veröffentlicht in:Scanning 2000-03, Vol.22 (2), p.91-92
Hauptverfasser: Kao, Fu-Jen, Lin, Bai-Ling, Cheng, Ping-Chin
Format: Artikel
Sprache:eng
Schlagworte:
Cells
Fluorescence
Laser damage
Laser pulses
Mercury vapor lamps
Photons
Plants (botany)
Solid state lasers
Ultraviolet radiation
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Zusammenfassung:In this study, two-photon excitation (TPE) under a microscope was employed to obtain the spectrum of chloroplast. It was found that the protoplasts from Arabidopsis thaliana may exhibit different fluorescence spectra depending on whether chloroplast exist in the cells. Further, it was observed that the fluorescence spectrum from the chloroplast is a time-dependent function of exposure.
ISSN:0161-0457