Resveratrol improves ex vivo expansion of CB‐CD34+ cells via downregulating intracellular reactive oxygen species level

Resveratrol improves ex vivo expansion of CB‐CD34+ cells via downregulating intracellular reactive oxygen species level

Intracellular reactive oxygen species (ROS) play important roles in the ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs). In this study, the effects of resveratrol (RES), on the ex vivo expansion of HSPCs were investigated by analyzing CD34+ cells expansion and biological functio...

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Veröffentlicht in:Journal of cellular biochemistry 2019-05, Vol.120 (5), p.7778-7787
Hauptverfasser: Tang, Chaochun, Zhang, Weiwei, Cai, Haibo, Ye, Zhaoyang, Zhang, Xu, Tan, Wensong
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
CD34 antigen
CD34+ cells
CD38 antigen
CD90 antigen
Cell culture
Cells (biology)
ex vivo expansion
Hematopoietic stem cells
Intracellular
Progenitor cells
Reactive oxygen species
Resveratrol
Scavenging
Semisolids
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Zusammenfassung:Intracellular reactive oxygen species (ROS) play important roles in the ex vivo expansion of hematopoietic stem and progenitor cells (HSPCs). In this study, the effects of resveratrol (RES), on the ex vivo expansion of HSPCs were investigated by analyzing CD34+ cells expansion and biological functions, with the objective to optimize ex vivo culture conditions for CD34 + cells. Among the five tested doses (0, 0.1, 1, 10, 20, and 50 μM), 10 μM RES was demonstrated to be the most favorable for ex vivo CD34 + cells expansion. In the primary cultures, 10 μM RES favored higher expansion folds of CD34 + cells, CD34 +CD38 − cells, and colony‐forming units (CFUs) ( P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28052