Base-Pair Formation of Self-Organizing RNA Amphiphiles within Two Dimensions

In an attempt to get a better insight in specific base pairing by self-organization of DNA or RNA, the synthesis of a complete set of monoalkyl ribonucleotides is described. The thermodynamic phase and mixing properties of these self-organizing RNA amphiphiles were analyzed within two dimensions using film balance technique. The pure compounds form stable monolayers at the air−water interface on a physiological buffer. In analogy to base-paired RNA or DNA in solution, the monolayers were mostly stabilized by complexing the phosphate groups by calcium or magnesium ions. By use of mixtures of RNA amphiphiles, specific recognition and base-pair formation of monoalkyl phospho adenosine/uridine were demonstrated.