Protective effects of trehalose on frozen-thawed ovarian granulosa cells of cattle

Protective effects of trehalose on frozen-thawed ovarian granulosa cells of cattle

In this study, trehalose was investigated for its cryoprotective effects on ovarian granulosa cells (bGCs) of cattle. Five concentrations of trehalose at 0, 0.2, 0.4, 0.6 and 0.8 mol/L were added to the cryopreservation medium of bGCs, and the effects on the quality of frozen-thawed bGCs were assess...

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Veröffentlicht in:Animal reproduction science 2019-01, Vol.200, p.14-21
Hauptverfasser: Zheng, Y.X., Ma, L.Z., Liu, S.J., Zhang, C.T., Meng, R., Chen, Y.Z., Jiang, Z.L.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Bovine
Cattle
Cells, Cultured
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Cytoprotection - drug effects
Female
Freezing
Frozen-thawed
Granulosa Cells
Ovary - drug effects
Trehalose
Trehalose - pharmacology
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Zusammenfassung:In this study, trehalose was investigated for its cryoprotective effects on ovarian granulosa cells (bGCs) of cattle. Five concentrations of trehalose at 0, 0.2, 0.4, 0.6 and 0.8 mol/L were added to the cryopreservation medium of bGCs, and the effects on the quality of frozen-thawed bGCs were assessed. The results indicate that the use of cryopreservation medium containing 0.2 and 0.4 mol/L of trehalose resulted in a greater rate of bGC viability compared to those of other groups (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2018.11.005