Cell behavior on the alginate-coated PLLA/PLGA scaffolds
Here, we investigated the effect of preparation temperature and alginate-coating on L929 fibroblast behavior on lyophilized microporous PLLA/PLGA (95:5, w/w) scaffolds. The lower freezing temperature used during lyophilization (−80 °C) resulted in smaller pores (around 50 μm) and higher compressive...
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Veröffentlicht in: | International journal of biological macromolecules 2019-03, Vol.124, p.444-450 |
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description | Here, we investigated the effect of preparation temperature and alginate-coating on L929 fibroblast behavior on lyophilized microporous PLLA/PLGA (95:5, w/w) scaffolds. The lower freezing temperature used during lyophilization (−80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) than those prepared at the higher temperature (−20 °C) (pore size: 120 μm, compressive modulus: 600 kPa) (p |
doi_str_mv | 10.1016/j.ijbiomac.2018.11.169 |
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The lower freezing temperature used during lyophilization (−80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) than those prepared at the higher temperature (−20 °C) (pore size: 120 μm, compressive modulus: 600 kPa) (p < 0.01). Cell proliferation was significantly lower on the alginate-coated scaffolds (p < 0.05), probably due to weak cell adhesion on alginate, rapid degradation/dissolution of the alginate hydrogel (40% weight loss after 2 weeks of incubation) (p < 0.05), which resulted in loss of material and cells, and the decrease in the pH (p < 0.05), which probably resulted in decreased cell metabolic activity. Cells tended to get less round on the scaffolds prepared at −20 °C, which had lower compressive modulus and larger pores, and upon coating with alginate, which resulted in a hydrophilic surface that had lower stiffness. When the scaffolds had closer stiffness to the cells, the cells tended to get more branched. 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The lower freezing temperature used during lyophilization (−80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) than those prepared at the higher temperature (−20 °C) (pore size: 120 μm, compressive modulus: 600 kPa) (p < 0.01). Cell proliferation was significantly lower on the alginate-coated scaffolds (p < 0.05), probably due to weak cell adhesion on alginate, rapid degradation/dissolution of the alginate hydrogel (40% weight loss after 2 weeks of incubation) (p < 0.05), which resulted in loss of material and cells, and the decrease in the pH (p < 0.05), which probably resulted in decreased cell metabolic activity. Cells tended to get less round on the scaffolds prepared at −20 °C, which had lower compressive modulus and larger pores, and upon coating with alginate, which resulted in a hydrophilic surface that had lower stiffness. When the scaffolds had closer stiffness to the cells, the cells tended to get more branched. 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The lower freezing temperature used during lyophilization (−80 °C) resulted in smaller pores (around 50 μm) and higher compressive modulus (1500 kPa) than those prepared at the higher temperature (−20 °C) (pore size: 120 μm, compressive modulus: 600 kPa) (p < 0.01). Cell proliferation was significantly lower on the alginate-coated scaffolds (p < 0.05), probably due to weak cell adhesion on alginate, rapid degradation/dissolution of the alginate hydrogel (40% weight loss after 2 weeks of incubation) (p < 0.05), which resulted in loss of material and cells, and the decrease in the pH (p < 0.05), which probably resulted in decreased cell metabolic activity. Cells tended to get less round on the scaffolds prepared at −20 °C, which had lower compressive modulus and larger pores, and upon coating with alginate, which resulted in a hydrophilic surface that had lower stiffness. When the scaffolds had closer stiffness to the cells, the cells tended to get more branched. 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title | Cell behavior on the alginate-coated PLLA/PLGA scaffolds |
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