Continuous cell separation using novel magnetic quadrupole flow sorter

A laboratory prototype of a flow cell sorter based on magnetic quadrupole field was built and evaluated. The magnetic force acting on magnetically labeled cells in such a field has a `centrifugal’ character which provides a basis for the design of a continuous separation process. The sorter was test...

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Veröffentlicht in:	Journal of magnetism and magnetic materials 1999, Vol.194 (1), p.224-230
Hauptverfasser:	Zborowski, Maciej, Sun, Liping, Moore, Lee R, Stephen Williams, P, Chalmers, Jeffrey J
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biological and medical sciences Cell separation Cells Colloids Diverse techniques Fundamental and applied biological sciences. Psychology Human lymphocytes Immunomagnetic separation Magnetic colloid Magnetic fields Magnetite Molecular and cellular biology Quadrupole field
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Beschreibung
Zusammenfassung:	A laboratory prototype of a flow cell sorter based on magnetic quadrupole field was built and evaluated. The magnetic force acting on magnetically labeled cells in such a field has a `centrifugal’ character which provides a basis for the design of a continuous separation process. The sorter was tested on a model cell system of human peripheral lymphocytes labeled with magnetic colloid.
ISSN:	0304-8853
DOI:	10.1016/S0304-8853(98)00581-2