High-depth fluorescence imaging using a two-photon FRET system for mitochondrial pH in live cells and tissues

We developed a fluorescent pH probe ( 1 ) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2018-12, Vol.54 (96), p.13531-13534
Hauptverfasser:	Chang, Min Jung, Kim, Kyutae, Park, Kyun Seob, Kang, Ji Su, Lim, Chang Su, Kim, Hwan Myung, Kang, Chulhun, Lee, Min Hee
Format:	Artikel
Sprache:	eng
Schlagworte:	Emission Fluorescence Fluorescence Resonance Energy Transfer - methods Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Fluorescent Dyes - radiation effects HeLa Cells Humans Hydrogen-Ion Concentration Microscopy, Confocal - methods Mitochondria Mitochondria - metabolism Naphthalimides - chemical synthesis Naphthalimides - chemistry Naphthalimides - radiation effects NMR Nuclear magnetic resonance Photons Piperazines - chemical synthesis Piperazines - chemistry Piperazines - radiation effects Rhodamine Rhodamines - chemical synthesis Rhodamines - chemistry Rhodamines - radiation effects
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	We developed a fluorescent pH probe ( 1 ) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine. It exhibited a pH-dependent reversible and fast ratiometric fluorescence change in the rhodamine emission. Probe 1 was applied to image the pH perturbations of mitochondria in living cells and tissues. We developed a fluorescent pH probe ( 1 ) capable of two-photon excitation and far-visible-emission based on FRET, composed of naphthalimide-piperazine-rhodamine.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c8cc07934j