Genetic analysis among selected vernonia lines through seed oil content, fatty acids and RAPD DNA markers

Vernonia (Vernonia galamensis) is a new potential industrial oilseed crop. The seeds of this crop contain unusual naturally epoxidised fatty acids which are used in the production of various industrial products. The objective of this study was to evaluate and select vernonia lines in Limpopo provinc...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:African journal of biotechnology 2010-01, Vol.9 (2), p.117-122
Hauptverfasser: Ramalema, S P, Shimelis, H, Ncube, I, Kunert, K K, Mashela, P W
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Vernonia (Vernonia galamensis) is a new potential industrial oilseed crop. The seeds of this crop contain unusual naturally epoxidised fatty acids which are used in the production of various industrial products. The objective of this study was to evaluate and select vernonia lines in Limpopo province through seed oil content, fatty acid content and RAPD DNA markers. Significant differences were observed for the content of seed oil (22.4 - 29.05%), vernolic acid (73.09 - 76.83%), linoleic acid (13.02 - 14.05%), oleic acid (3.77 - 5.28%), palmitic acid (2.48 - 2.98%) and stearic acid (2.26 - 2.75%). Among the 13 RAPD DNA primers screened, primer OPA sub(10) amplified DNA samples and resulted in 4 distinct groupings among tested lines. Four promising lines were selected; Vge-16, Vge-20, Vge-27 and Vge-32 using seed oil content, fatty acids and RAPD markers. The lines will be used for strategic breeding of vernonia as an alternative industrial oil crop in Limpopo province of South Africa or other similar environments.
ISSN:1684-5315
1684-5315