Production and Characterization of an Alkaline Thermostable Crude Lipase from an Isolated Strain of Bacillus cereus C sub(7)

A bacterial strain isolated from spoiled coconut and identified as Bacillus cereus was found capable of producing alkaline thermostable extracellular lipase. Optimum temperature, time, and pH for enzyme substrate reaction were found to be 60C, 10min, and 8.0 respectively. Common surfactants except T...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Applied biochemistry and biotechnology 2009-10, Vol.159 (1), p.142-154
Hauptverfasser: Dutta, Sanghamitra, Ray, Lalitagauri
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A bacterial strain isolated from spoiled coconut and identified as Bacillus cereus was found capable of producing alkaline thermostable extracellular lipase. Optimum temperature, time, and pH for enzyme substrate reaction were found to be 60C, 10min, and 8.0 respectively. Common surfactants except Triton X 100 and cetyltrimethylammonium bromide have no or very little inhibitory effects on enzyme activity. The enzyme was found to be stable in presence of oxidizing agents and protease enzyme. The maximum lipase production was achieved at 30-33C, pH8.0 on 24h of fermentation using 50ml medium in a 250-ml Erlenmeyer flask. The superior carbon and nitrogen sources for lipase production were starch (2%) and ammonium sulfate (nitrogen level 21.2mg/100ml), peptone (nitrogen level 297mg/100ml), and urea (nitrogen level 46.62mg/100ml) in combination, respectively. The maximum enzyme activity obtained was 33c0.567IU/ml.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-009-8543-x