Production and Characterization of an Alkaline Thermostable Crude Lipase from an Isolated Strain of Bacillus cereus C sub(7)
A bacterial strain isolated from spoiled coconut and identified as Bacillus cereus was found capable of producing alkaline thermostable extracellular lipase. Optimum temperature, time, and pH for enzyme substrate reaction were found to be 60C, 10min, and 8.0 respectively. Common surfactants except T...
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Veröffentlicht in: | Applied biochemistry and biotechnology 2009-10, Vol.159 (1), p.142-154 |
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Sprache: | eng |
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Zusammenfassung: | A bacterial strain isolated from spoiled coconut and identified as Bacillus cereus was found capable of producing alkaline thermostable extracellular lipase. Optimum temperature, time, and pH for enzyme substrate reaction were found to be 60C, 10min, and 8.0 respectively. Common surfactants except Triton X 100 and cetyltrimethylammonium bromide have no or very little inhibitory effects on enzyme activity. The enzyme was found to be stable in presence of oxidizing agents and protease enzyme. The maximum lipase production was achieved at 30-33C, pH8.0 on 24h of fermentation using 50ml medium in a 250-ml Erlenmeyer flask. The superior carbon and nitrogen sources for lipase production were starch (2%) and ammonium sulfate (nitrogen level 21.2mg/100ml), peptone (nitrogen level 297mg/100ml), and urea (nitrogen level 46.62mg/100ml) in combination, respectively. The maximum enzyme activity obtained was 33c0.567IU/ml. |
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ISSN: | 0273-2289 1559-0291 |
DOI: | 10.1007/s12010-009-8543-x |