Alginate microparticles loaded with basic fibroblast growth factor induce tissue coverage in a rat model of myelomeningocele

We sought to develop a minimally invasive intra-amniotic therapy for prenatal treatment of myelomeningocele (MMC) in an established rat model. Time-dated pregnant rats were gavage-fed retinoic acid to induce MMC. Groups received intraamniotic injections at E17.5 with alginate particles loaded with f...

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Veröffentlicht in:Journal of pediatric surgery 2019-01, Vol.54 (1), p.80-85
Hauptverfasser: Farrelly, James S, Bianchi, Anthony H, Ricciardi, Adele S, Buzzelli, Gina L, Ahle, Samantha L, Freedman-Weiss, Mollie R, Luks, Valerie L, Saltzman, W Mark, Stitelman, David H
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Sprache:eng
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Zusammenfassung:We sought to develop a minimally invasive intra-amniotic therapy for prenatal treatment of myelomeningocele (MMC) in an established rat model. Time-dated pregnant rats were gavage-fed retinoic acid to induce MMC. Groups received intraamniotic injections at E17.5 with alginate particles loaded with fluorescent dye, basic fibroblast growth factor (Alg-HSA-bFGF), fluorescently tagged albumin (Alginate-BSA-TR), free bFGF, blank alginate particles (Alg-Blank), or PBS. Groups were analyzed at 3 h for specific particle binding or at term (E21) to determine MMC coverage. Alginate microparticles demonstrated robust binding to the MMC defect 3 h after injection. Of those specimens analyzed at E21, 150 of 239 fetuses (62.8%) were viable. Moreover, 18 of 61 (30%) treated with Alg-HSA-bFGF showed evidence of soft tissue coverage compared to 0 of 24 noninjected (P = 0.0021), 0 of 13 PBS (P = 0.0297), and 0 of 42 free bFGF (P = P 
ISSN:0022-3468
1531-5037
DOI:10.1016/j.jpedsurg.2018.10.031