AIP regulates stability of Aurora-A at early mitotic phase coordinately with GSK-3b

Glycogen synthase kinase-3 (GSK-3b) regulates microtubule dynamics and cellular polarity through phosphorylating various microtubule associating proteins and plus-end tracking proteins. Although it was also reported that GSK-3b is inactivated by protein kinase B at the spindle poles, functions and targets of GSK-3b in the mitotic phase are unknown. Here, we identified Aurora-A-interacting protein (AIP), a negative regulator of Aurora-A, as a binding partner of GSK-3b. AIP was colocalized with Aurora-A and GSK-3b to the spindle poles in metaphase, and its depletion in cells stabilized and activated Aurora-A in early mitotic phase and caused mitotic cell arrest. Treatment of the cells with a GSK-3b inhibitor reduced the protein level of Aurora-A and this reduction was suppressed by AIP knockdown. AIP was phosphorylated by GSK-3b, and an AIP mutant in which the GSK-3b phosphorylation site was mutated could bind and downregulate Aurora-A more efficiently. These results suggest that GSK-3b modulates the early mitotic Aurora-A level through binding and phosphorylating AIP.Oncogene (2008) 27, 4478-4487; doi:10.1038/onc.2008.92; published online 7 April 2008