Kaolin particle film modulates morphological, physiological and biochemical olive tree responses to drought and rewatering

Regarding the foreseeing climate change is reasonable to expect harmful consequences to olive tree (Olea europaea L.), an iconic species of Mediterranean region. Thus, the selection of practices that allow a better drought resistance and recovery capacity needs the immediate attention of scientific community. This study evaluates the strategies adopted by young potted olive trees, subjected to three cycles of drought and rewatering, in the presence of a reflective clay, kaolin (KL). The results demonstrated that KL induced shade-related leaf structural changes and was effective in keeping leaf water status during the most stressful periods. In general, photosynthetic activity of sprayed plants was improved by the alleviation of drought-induced stomatal and non-stomatal limitations. Moreover, during stress imposition sprayed leaves showed reduced oxidative damages, allowing lower investment in antioxidant defences. Furthermore, sprayed plants also had lower nighttime water losses due to inferior nighttime stomatal conductance, and are able to maintain higher respiration rates. Upon rewatering, the shaded effect conferred by KL limited gas exchange restauration, but improved the plants’ capacity to restore the metabolic functions. In spite of the induced physiological and biochemical changes, no significant differences were found in whole-plant water use efficiency and plant biomass accumulation, possibly by the attenuation of photosynthesis restauration during the recovery events. In conclusion, the changes induced by KL might be beneficial under severe conditions, as on realistic Mediterranean field environments. •Kaolin (KL) induces shade-related leaf characteristics.•KL improves water status and reduces nighttime water losses during drought.•KL improves photosynthetic capacity and reduces oxidative damages during drought.•KL limits gas exchange restauration after stress relief.•KL improves the capacity to restore the metabolic functions upon rewatering.