Unreliability of Results of PCR Detection of Helicobacter pylori in Clinical or Environmental Samples

The aim of this study was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pylori in gastric biopsy specimens and salivary samples. Detection limits of the 26 PCR primer pairs previously described for detection of H. pylori DNA in clinical samples were de...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-03, Vol.47 (3), p.738-742
Hauptverfasser:	Sugimoto, Mitsushige, Wu, Jeng-Yih, Abudayyeh, Suhaib, Hoffman, Jill, Brahem, Hajer, Al-Khatib, Khaldun, Yamaoka, Yoshio, Graham, David Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriology Biological and medical sciences DNA Primers - genetics Environmental Microbiology False Positive Reactions Fundamental and applied biological sciences. Psychology Gastric Mucosa - microbiology Helicobacter Infections - diagnosis Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - genetics Helicobacter pylori - isolation & purification Humans Microbiology Miscellaneous Polymerase Chain Reaction - methods Saliva - microbiology Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	The aim of this study was to compare published Helicobacter pylori primer pairs for their ability to reliably detect H. pylori in gastric biopsy specimens and salivary samples. Detection limits of the 26 PCR primer pairs previously described for detection of H. pylori DNA in clinical samples were determined. Sensitivity and specificity were determined using primers with detection limits of
ISSN:	0095-1137 1098-660X
DOI:	10.1128/JCM.01563-08