Comparison of Cefoxitin Disc Diffusion Test, Oxacillin Screen Agar, and PCR for mecA Gene for Detection of MRSA
Cefoxitin is a potent inducer of the mecA regulatory system. It is being recommended for detection of methicillin resistance in Staphylococcus aureus (MRSA) when using disk diffusion testing. The aim of our study was to evaluate the efficacy of cefoxitin disc diffusion test to characterize MRSA and...
Gespeichert in:
Veröffentlicht in: | Indian journal of medical microbiology 2009-01, Vol.27 (1), p.27-29 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Cefoxitin is a potent inducer of the mecA regulatory system. It is
being recommended for detection of methicillin resistance in
Staphylococcus aureus (MRSA) when using disk diffusion testing. The
aim of our study was to evaluate the efficacy of cefoxitin disc
diffusion test to characterize MRSA and compare it with oxacillin agar
screening and detection of mecA gene by PCR. Materials and Methods:
Fifty strains of S. aureus isolated from clinical samples were used in
the study. Routine antibiotic susceptibility testing was performed
including oxacillin disk. Oxacillin screen agar plates with 4% NaCl and
6 µg/ml of oxacillin were inoculated and interpreted as per
standard guidelines. Cefoxitin disc diffusion test was performed using
30 µg disc and zone sizes were measured. PCR for amplification of
the mecA gene was performed. Results: Out of the 50 isolates, 28
were found to be methicillin resistant by oxacillin disc diffusion
test, 30 were resistant by oxacillin screen agar method, and 32 were
resistant with cefoxitin disc diffusion. For these 32 isolates mecA
gene was positive. Conclusion: Results of cefoxitin disc diffusion
test is in concordance with the PCR for mecA gene. Thus, the test can
be an alternative to PCR for detection of MRSA in resource constraint
settings. |
---|---|
ISSN: | 0255-0857 1998-3646 |
DOI: | 10.1016/S0255-0857(21)01748-5 |