Algoriphagus olei sp. nov., isolated from oil-contaminated soil

1 College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC 2 Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC 3 Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität Wien, Vienna, Austria 4 Institut für Angewandte Mikrobiologie, Universität Giessen, Giessen, Germany 5 Yenepoya Research Center, Yenepoya University, University Road, Deralakatte, Mangalore, 575018, Karnataka, India Correspondence Peter Kämpfer peter.kaempfer{at}umwelt.uni-giessen.de A motile, Gram-negative, pinkish red-pigmented bacterium (strain CC-Hsuan-617 T ) was isolated from an oil-contaminated soil near an oil refinery located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis showed that strain CC-Hsuan-617 T clustered with Algoriphagus mannitolivorans (97.5 % sequence similarity), Algoriphagus aquatilis (98.0 %) and Algoriphagus boritolerans (97.5 %), followed by Algoriphagus ornithinivorans (97.1 %) and Algoriphagus alkaliphilus (97.1 %). The fatty acid profile of the novel strain was slightly different from those reported for recognized Algoriphagus species. The quinone system contained menaquinone MK-7 as the predominant component. The major polar lipids were phosphatidylethanolamine, an unknown polar lipid, an unknown phospholipid and an unknown aminophospholipid. The main cell polyamine was sym -homospermidine; lesser amounts of spermine and spermidine were also found. The results of DNA–DNA hybridization, and physiological and biochemical tests allowed the genotypic and phenotypic differentiation of strain CC-Hsuan-617 T from recognized Algoriphagus species. Strain CC-Hsuan-617 T is thus considered to represent a novel species of the genus Algoriphagus , for which the name Algoriphagus olei sp. nov. is proposed. The type strain is CC-Hsuan-617 T (=BCRC 17886 T =CCUG 57471 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain CC-Hsuan-617 T is FJ231215 .