Enhanced reversible redox activity of hemin on cellulose microfiber integrated reduced graphene oxide for H2O2 biosensor applications

[Display omitted] •A novel H2O2 biosensor based on hemin/RGO-CMF composite.•Vitamin C assisted synthesis of RGO-CMF composite.•The RGO-CMF improve the hemin redox electrochemistry by 6 folds compared to RGO.•High sensitivity, lower LOD with a wider linear response range of H2O2 has been achieved.•The sensor can be directly used for the sensitive detection of H2O2 in food and environmental samples. In recent years, the carbohydrate polymers incorporated composite materials have shown significant interest in the bioanalytical chemistry due to their enhanced catalytic performances of various enzymes or mimics. This paper reports the fabrication of novel H2O2 biosensor using a hemin immobilized reduced graphene oxide-cellulose microfiber composite (hemin/RGO-CMF). The RGO-CMF composite was prepared by the reduction of GO-CMF composite using vitamin C as a reducing agent. Various physio-chemical methods have applied for the characterization of RGO-CMF composite. Cyclic voltammetry results revealed that the hemin/RGO-CMF composite shows a better redox electrochemical behavior than hemin/RGO and hemin/GO-CMF. Under optimized conditions, the hemin/RGO-CMF composite exhibit a linear response to H2O2 in the concentration range from 0.06 to 540.6 μM with the lower detection limit of 16 nM. The sensor also can able to detect the H2O2 in commercial contact lens solution and milk samples with functional recovery, which authenticates the potential ability in practical sensors.